Biology Department Biology Department  


Annie Héroux

Brookhaven National Laboratory
Bldg. 463 - P.O. Box 5000
Upton, NY 11973-5000

Phone: (631) 344-4454
Fax: (631) 344-2741
The Group:
Stuart Myers (631) 344-5726

Annie Héroux is a beam line scientist in the Macromolecular Crystallography Research Resource (PXRR) which provides facilities and support at the National Synchrotron Light Source for the benefit of outside and in-house investigators.   The PXRR is supported by the NIH's National Center for Research Resources and the DOE Office of Biological and Environmental Research in its mission to create optimal facilities and environments for macromolecular structure determination by synchrotron X-ray diffraction.   With a staff of about 24, the PXRR innovates new access modes such as FedEx crystallography, builds new facilities, currently on the X25 undulator, advances automation, develops remote participation software, collaborates with outside groups, teaches novice users, and supports visting investigators with 7day, 20 hours staff coverage.

Research Interests and Institutional Responsibilities:

Beam Line Scientist at X25:   As the beam line scientist at the macromolecular crystallography beam line X25, I oversee, test, and apply experimental techniques and structure determination methods that benefit the visiting scientists and the PXRR. A current focus is the shaping of the PXdb from the experimentor's point of view. The PXdb is our data base program that captures all aspects of data collection at the PXRR beamlines from project definition, to detailed journaling of data collections.

Mail-in Crystallography:. In addition, I manage the PXRR Mail-in Crystallography Program. This team includes Howard Robinson and Alexei Soares, as well as our PX support staff in carrying out an extensive remote participation data collection effort.


Selected Publications

Note: The PDB files below can be viewed with
  • Héroux A., Bozinovski D.M., Valley M.P., Fitzpatrick P.F.. and Orville A.M.
    Crystal Structures of Intermediates in the Nitroalkane Oxidase Reaction
    Biochemistry, 48(15):3407-3416 (2009).  PubMed
    PDB files: 3D9D, 3D9E, 3D9F, 3D9G ,   Jmol viewer
  • Bowley S.R., Merenbloom B.K., Okumura N., Betts L., Heroux A., Gorkun O.V. and Lord S.T.
    Polymerization-defective fibrinogen variant γD364A binds knob “A” peptide mimic.
    Biochemistry, 47(33):8607-8613 (2008).  PubMed  
  • Garst A.D., Heroux A., Rambo R.P. and Batey R.T.
    Crystal structure of the lysine riboswitch regulatory mRNA element.
    Journal of Biological Chemistry, 283(33):22347-22351 (2008).  PubMed
  • Gonciarz M.D., Whitby F.G., Eckert D.M., Kieffer C., Heroux A., Sundquist W.I. and Hill C.P.
    Biochemical and structural studies of yeast Vps4 oligomerization.
    Journal of Molecular Biology, 384(4):878-895 (December, 2008).   PubMed
  • Hall B.M., Roberts S.A., Heroux A. and Cordes M.H.
    Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA.
    J Mol Biol., 375(3):802-811 (2008).   PubMed  
  • Schubert H.L., Phillips J.D., Heroux A. and Hill C.P.
    Structure and mechanistic implications of a uroporphyrinogen III synthase--product complex.
    Biochemistry, 47(33):8648-8655 (2008).  PubMed
  • Spoonamore J.E., Roberts S.A., Heroux A. and Bandarian V.
    Structure of a 6 pyruvoyltetrahydropterin synthase homolog from Streptomyces coelicolor.
    Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F64(Pt 10):875-879 (2008).  PubMed
  • Pallan P.S., Ittig D., Heroux A., Wawrzak Z., Leumann C.J. and Egli M.
    Crystal structure of tricyclo-DNA: an unusual compensatory change of two adjacent backbone torsion angles.
    Chemical Communications (Cambridge), 2008(7):883-885 (2008).  PubMed  
  • Petrie E.J., Clements C.S., Lin J., Sullivan L.C., Johnson D., Huyton T., Heroux A., Hoare H.L., Beddoe T., Reid H.H., Wilce M.C.J., Brooks A.G. and Rossjohn J.
    CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence.
    Journal of Experimental Medicine, 205(3):725-735 (2008).  PubMed  
  • VanDemark A.P., Xin H., McCullough L., Rawlins R., Bentley S., Heroux A., Stillman D.J., Hill C.P. and Formosa T.
    Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits.
    Journal of Biological Chemistry, 283(8):5058-5068 (2008).  PubMed  
  • Yang K., Zhang L., Xu T., Heroux A. and Zhao R.
    Crystal structure of the β-finger domain of Prp8 reveals analogy to ribosomal proteins.
    Proceedings of the National Academy of Sciences USA, 105(37):13817-13822 (2008).  PubMed
  • Fitzpatrick P.F., Bozinovski D.M., Heroux A., Shaw P.G., Valley M.P. and Orville A.M.
    Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase.
    Biochemistry, 46(48):13800-13808 (2007).   PubMed  
  • Renner M.W., Bochot C., Heroux A., Mansury D. and Battioni P.
    Structural, electrochemical and spectroscopic properties of a class of dodecasubstituted iron porphyrins bearing four positive charges close to metal.
    Eur J Inorg Chem., 2426-2433 (2007).
  • VanDemark A.P., Kasten M.M., Ferris E., Heroux A., Hill C.P. and Cairns B.R.
    Autoregulation of the rsc4 tandem bromodomain by Gcn5 acetylation.
    Mol Cell, 27(5):817-828 (2007).   PubMed  
  • Vandemark A.P., Xin H., McCullough L., Rawlins R., Bentley S., Heroux A., Stillman D.J., Hill C.P. and Formosa T.
    Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits.
    J Biol Chem., 283(8):5058-5068 (2007).   PubMed  
  • Welch B.D., Vandemark A.P., Heroux A., Hill C.P. and Kay M.S.
    Potent D-peptide inhibitors of HIV-1 entry.
    Proc Natl Acad Sci USA, 104(43):16828-16833 (2007).   PubMed  
  • Zhang L., Shen J., Guarnieri M.T., Heroux A., Yang K. and Zhao R.
    Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants.
    Protein Sci., 16(6):1024-31 (2007).  PubMed  
  • Gallagher D.T., Smith N.N., Kim S.K., Heroux A., Robinson H. and Reddy P.T.
    Structure of the class IV adenylyl cyclase reveals a novel fold.
    J Mol Biol., 362:114-122 (2006).   PubMed
    PDB file: 2FJT   Jmol viewer
  • Robinson H., Soares A.S., Becker M., Sweet R. and Heroux A.
    Mail-in crystallography program at Brookhaven National Laboratory's National Synchrotron Light Source.
    Acta Cryst., D62(11):1336-1339 (2006).   PubMed   Full Text
  • Skinner J.M., Cowan M., Buono R., Nolan W., Bosshard H., Robinson H.H., Heroux A., Soares A.S., Schneider D.K. and Sweet R.M.
    Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database
    Acta Cryst., D62(11):1340-1347 (2006).   PubMed   Full Text
  • Reyes-Turcu F.E., Horton J.R., Mullally J.E., Heroux A., Cheng X., and Wilkinson K.D.
    The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin.
    Cell, 124(6):1197-1208 (2006).   PubMed
    PDB files: 2G432G45   Jmol viewer
  • VanDemark A.P., Blanksma M., Ferris E., Heroux A., Hill C.P. and Formosa T.
    The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition.
    Mol Cell, 22:363-374 (2006).   PubMed
    PDB files: 2GCL,   2GCJ   Jmol viewer
  • Auperin T.C., Bolduc G.R., Baron M.J., Héroux A., Filman D.J., Madoff L.C., Hogle J.M.
    Crystal structure of the N-terminal domain of the group B Streptococcus alpha C protein.
    J Biol Chem., 280(18):18245-18252 (2005).   PubMed   Full Text
    PDB file: 1YWM   Jmol viewer
  • Davison P.A., Schubert H.L., Reid J.D., Iorg C.D., Héroux A., Hill C.P. and Hunter C.N.
    Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis.
    Biochemistry, 44(21):7603-7612 (2005).   PubMed
    PDB files: 1Z3X,   1Z3Y   Jmol viewer
  • Loftin I.R., Franke S., Roberts S.A., Weichsel A., Héroux A., Montfort W.R., Rensing C., McEvoy M.M.
    A novel copper-binding fold for the periplasmic copper resistance protein CusF.
    Biochemistry, 44(31):10533-10540 (2005).   PubMed
    PDB file: 1ZEQ   Jmol viewer
  • Gopalan G., He Z., Balmer Y., Romano P., Gupta R., Héroux A., Buchanan B.B., Swaminathan K. and Luan S.
    Structural analysis uncovers a role for redox in regulating FKBP13, an immunophilin of the chloroplast thylakoid lumen.
    Proc Natl Acad Sci USA, 101(38):13945-13950 (2004).   PubMed   Full Text
    PDB file: 1U79   Jmol viewer
  • Smith N., Mayhew M., Holden M.J., Kelly H., Robinson H., Héroux A., Vilker V.L. and Gallagher D.T.
    Structure of C73G putidaredoxin from Pseudomonas putida.
    Acta Cryst., D60(5):816-822 (2004).   PubMed
  • Smith N., Mayhew M., Robinson H., Héroux A., Charlton D., Holden M.J. and Gallagher D.T.
    Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus.
    Acta Cryst., D59(12):2328-2331 (2003).   PubMed
  • Soares A.S., Caspar D.L., Weckert E., Héroux A., Hölzer K., Schroer K., Zellner J., Schneider D., Nolan W. and Sweet R.M.
    Three-beam interference is a sensitive measure of the efficacy of macromolecular refinement techniques
    Acta Cryst., D59(10):1716-1724 (2003).   PubMed   Full Text
  • Klon E., Héroux A., Ross L.J., Pathak V., Johnson C.A., Piper R.J. and Borhani D.W.
    Atomic Resolution Structures of Human Dihydrofolate Reductase Complexed with NADPH and Two Lipophilic Antifolates at 1.09 A and 1.05 A Resolution.
    J Mol Biol., 320(3):677-693 (2002).   PubMed
    PDB files 1KMS,   1KMV   Jmol viewer
  • Héroux A., White E.L., Ross L.J., Kuzin A.P. and Borhani D.W.
    Substrate Deformation in a Hypoxanthine-Guanine Phosphoribosyltransferase Ternary Complex: The Structural Basis for Catalysis.
    Structure Fold Des., 8(12):1309-1318 (2000).   PubMed
    PDB file 1FSG   Jmol viewer
  • Héroux A., White E.L., Ross L.J., and Borhani D.W.
    Crystal Structures of the Toxoplasma gondii Hypoxanthine-Guanine Phosphoribosyltransferase-GMP and IMP Complexes: Comparison of Purine Binding Interactions with the XMP Complex
    Biochemistry, 38(44):14485-14494 (1999).   PubMed
    PDB files 1QK4,   1QK3   Jmol viewer
  • Héroux A., White E.L., Ross L.J., Davis R.L. and Borhani D.W.
    Crystal Structure of Toxoplasma gondii Hypoxanthine-Guanine Phosphoribosyltransferase with XMP, Pyrophosphate and Two Mg2+ Ions Bound: Insights Into the Catalytic Mechanism.
    Biochemistry, 38(44):14495-14506 (1999).   PubMed
    PDB file 1QK5   Jmol viewer
  • D.W. Borhani, Héroux A., and Piper J.R.
    Ultra-high Resolution Crystal Structure of Human Dihydrofolate Reductase Bound to a Lipophilic Antifolate
    Stanford Synchrotron Radiation Laboratory, 1997 Annual Report.
  • A. Héroux and  Brisse F.
    Acta Cryst, C53:1318-1320 (1997).
  • A. Héroux and Brisse F.
    Acta Cryst., C52:224-226 (1996).
  • M. Belletête, Brisse F., Durocher G., Gravel D., Héroux A. and Popowyck A.
    J Mol Structure, 297:63-80 (1993).
  • M. Lachapelle, Belletête M., Poulin M., Godbout N., LeGrand F., Héroux A., Brisse F. and Durocher G.
    J Phys Chem., 95:9764-72 (1991).


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Last Modified: June 16, 2009
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