Dieter K. Schneider

Brookhaven National Laboratory
Bldg. 463 - P.O. Box 5000
Upton, NY 11973-5000

Dieter Schneider is a co-principal investigator in the Macromolecular Crystallography Research Resource (PXRR) which provides facilities and support at the National Synchrotron Light Source for the benefit of outside and in-house investigators.   The PXRR is supported by the NIH's National Center for Research Resources and the DOE Office of Biological and Environmental Research in its mission to create optimal facilities and environments for macromolecular structure determination by synchrotron X-ray diffraction.   With a staff of about 25, the PXRR innovates new access modes such as FedEx crystallography, builds new facilities, currently on the X29 undulator, advances automation, develops remote participation software, collaborates with outside groups, teaches novice users, and supports visting investigators with 7day, 20 hours staff coverage.

Research Interests

Applications of neutron small-angle scattering for the structure determination of macromolecular complexes are the focus of this program. Development of scattering techniques, computational methods, and neutron instrumentation is pursued to maintain a vigorous outside user program on the H9B SANS Beamline at the High Flux Beam Reactor.

While the HFBR is shutdown, development of a neutron fiber diffraction camera is continued. A dual purpose instrument for the measurement of diffuse scattering and fibre diffraction was built jointly with the BNL Physics Department and is being tested at the National Institute of Standards and Technology. Neutron image plate detectors are used to record diffraction at a high resolution. The new capabilities will be applied by structural biologists who are deriving the organization of multicomponent macromolecular fibrous complexes, probing the conformational changes in contractile proteins, and determining intermolecular forces.

Additionaly, I am the beamline scientist at the NSLS beam line X26C, which is operated by a four-membered PRT including the BNL Biology Department, Cold Spring Harbor Laboratory, the State University of New York at Stony Brook, and the Georgia Research Alliance.

Neutron fibre diffraction pattern of a crystalline TMV specimen in a capillary
of 0.7 mm diameter recorded for 10 minutes at H9B.

Selected Publications

• Soares A.S., Schneider D.K., Skinner J.M., Cowan M., Buono R., Robinson H.H., Heroux A., Carlucci-Dayton M., Saxena A., and Sweet R.M.
  Remote access to the PXRR macromolecular crystallography facilities at the NSLS.
  Synchrotron Radiation News, 21(5):17-23 (September, 2008).
• Skinner J.M., Cowan M., Buono R., Nolan W., Bosshard H., Robinson H.H., Heroux A., Soares A.S., Schneider D.K. and Sweet R.M.
  Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database.
  Acta Cryst., D62(11):1340-1347 (2006).   PubMed   Full Text
• Shi W., Robinson H., Sullivan M., Abel D., Toomey J., Berman L.E., Lynch D., Rosenbaum G., Rakowsky G., Rock L., Nolan B., Shea-McCarthy G., Schneider D., Johnson E., Sweet R.M. and Chance M.R.
  Beamline X29: a novel undulator source for X-ray crystallography.
  J Synch Rad., 13:365-372 (2006).   PubMed   Full Text
• Robinson H.H., Shi W., Sullivan M., Nolan W., Schneider D.K., Berman L., Lynch D., Rock L., Rosenbaum G., Johnson E., Chance M.R. and Sweet R.M.
  An undulator beamline for protein crystallography at the NSLS: Commissioning and operation of X29.
  Synch Rad News, 18(5):27-31 (2005).   Full Text
• Soares A.S., Caspar D.L.D., Weckert E., Héroux A., Hölzer K., Schroer K., Zellner J., Schneider D., Nolan W. and Sweet R.M.
  Three beam interference is a sensitive measure of the efficacy of macromolecular refinement techniques.
  Acta Cryst, D59(10):1716-1724 (2003). PubMed  Full Text
• Schneider D.K., Chen S.H., Capel M.S., Hsiao B., Kostorz G., Pedersen J.S., Timmins P. and Wignall G.D.
  Issue Preface: XIth International Conference on Small-Angle Scattering (BNL, May 17-20, 1999).
  J Appl Cryst, 33:(3)(2000).
• Stone D.B., Timmins P.A., Schneider D.K., Krylova I., Ramos C.H.I., Reinach F.C. and Mendelson R.A.
  The Effect of Regulatory Ca++ on the in situ Structures of Troponin C and Troponin I: A Neutron Scattering Study.
  J Mol Biol., 281:689-704 (1998). PubMed.
• Imai M., Kato T. and Schneider D.
  Fluctuations and growth of a cubic network observed in a nonionic surfactant system.
  J Chem Phys, 106:9362-9371 (1997).
• Mendelson R.A., Schneider D.K. and Stone D.B.
  Conformations of Myosin Subfragment 1 ATPase Intermediates from Neutron and X-ray Scattering.
  J Mol Biol., 256:1-7 (1996). PubMed.
• Wu C., Quesada M.A., Schneider D.K., Farinato R., Studier F.W. and Chu B.
  Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: mesh sizes, separation and dispersion.
  Electrophoresis, 17:1103-1109 (1996). PubMed.
• Flanagan J.M., Wall J.S., Capel M.S., Schneider D.K. and Shanklin J.
  Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore.
  Biochemistry, 34:10910-10917 (1995). PubMed
• Graziano V., Gerchman S.E., Schneider D.K. and Ramakrishnan V.
  Histone H1 is located in the interior of the chromatin 30-nm filament.
  Nature, 368:351-354 (1994). PubMed
• Bivin D.B., Stone D.B., Schneider D.K. and Mendelson R.A.
  Cross-helix separation of tropomyosin molecules in acto-tropomyosin as determined by neutron scattering.
  Biophys J., 59:880-888 (1991). PubMed

Last Modified: March 26, 2010
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One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit, applied science and technology organization.

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