Biology Department Biology Department  

Scanning Transmission Electron Microscopy Facility

An Outside User Facility at the Biology Department

News Releases:

Simultaneous Nanoscale Imaging of Surface and Bulk Atoms
Details of Bacterial ‘Injection’ System Revealed
Structures of Important Plant Viruses Determined


Joseph S. Wall
James F. Hainfeld
Martha N. Simon
Frank E. Kito
Beth Yu Lin
tel: (631) 344-2912
tel: (631) 344-3367
tel: (631) 344-3372
tel: (631) 344-3372
tel: (631) 344-3372

BNL STEM Group (click to enlarge)

   Biology Department, Bldg 463
   Brookhaven National Lab
   Upton, NY 11973-5000

   fax: (631) 344-3407



STEM is a custom-built electron microscope optimized for imaging unstained biological
molecules with minimal radiation damage. The group at Brookhaven operates STEM as
a User Facility with partial DOE and partial support Fee-for-Service
(viewable with Abobe Reader).


STEM mass measurements can:

  • Determine stoichiometry and homogeneity of a complex
  • Compare modified or re-assembled vs. native structures
  • Determine filament symmetry from mass per unit length

In addition, STEM is the best instrument for visualizing small metal clusters used as
heavy atom labels.


Molecular Machines Flagella Virus Assembly
DNA/RNA/protein complexes Microtubules Heavy Atom Labels
Lipoproteins Prions Nano Particles
Alzhiemer’s Filaments Pili Nano Assemblies
Amyloids Filamentous Viruses  
Fibrin Spherical Viruses  


The role of the STEM and cryo-EM in structural biology is summarized by
Joe Wall in this 3.5 minute STEM Video clip viewable with Real Player.


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Last Modified: October 2, 2012
Please forward all questions about this site to: Kathy Folkers

Visit our Anniversary Website One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit, applied science and technology organization.

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