Biology Department Biology Department  

Scanning Transmission Electron Microscopy Facility


Comparison to Other Imaging Techniques (See Wiki of underlined terms)

Biological STEM: (BNL, Engel, Leapman, Ortega) emphasis on low-dose, dark field imaging of very thin specimens (~2nm resolution), quantitative imaging (mass mapping), specific heavy atom labels, low-Z negative staining.
Materials science STEM: (CFN, Pennycook, etc.) emphasis on high-dose, small probe (<0.1nm resolution with spherical aberration correction), electron energy loss spectroscopy (EELS) and micro-area diffraction.
Bright field TEM: emphasis on stained thin sections, complexes in high-Z negative stain. ~2nm resolution)
Dark field TEM: emphasis on thin specimens, similar to dark field STEM but 20x higher dose and non-linear image intensity.
Phase contrast TEM: emphasis on frozen-hydrated specimens (~0.5nm resolution with image averaging)
Secondary electron SEM (Scanning Electron Microscopy): emphasis on surface imaging of solid specimens after drying or freeze-fracturing followed by metal shadowing. (~2nm resolution)
AFM (Atomic Force Microscopy): emphasis on thin specimens on flat surfaces (mica), can be done in liquid (~2nm resolution)
STM (Scanning Tunneling Microscopy): emphasis on conducting properties of flat metal specimens (~0.2nm resolution).
X-ray Crystallography: emphasis on 3-D imaging of objects in crystals (~0.2 nm resolution in 3-D)
Confocal Optical Microscopy: emphasis on 3-D imaging of living tissue with specific fluorescent labels (~200nm resolution in 3-D and msec.)

 

Top of Page

Last Modified: June 12, 2009
Please forward all questions about this site to: Kathy Folkers


Visit our Anniversary Website One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit, applied science and technology organization.

Privacy and Security Notice  | Contact Web Services for help