Scanning Transmission Electron Microscopy Facility
Starting a Project
- Classify your interest into one or more of the following categories and check examples
a. Newly discovered complex, is it "real"? (Typically 3 specimens, 9 grids)
b. CryoEM or x-ray symmetry or unit cell is ambiguous (1 specimen, 3 grids)
c. You know subunit MW for components, but not stoichiometry for complex
(1 specimen, 3 grids)
d. You wish to compare assembled and native complexes (2 specimens, 6 grids)
e. Same as above but assembled with labeled subunit(s) (3 specimens, 9 grids)
f. You wish to follow a series of assembly steps or intermediates (N specimens, 3N grids)
- List: goal(s), sample physical description, concentration, buffer, stability (vs. time, salt, concentration, detergent
(usually a problem)) and time frame. Note that particulates and fibers with no UV absorption or staining on gels will be
perfectly visible in STEM specimens and may complicate analysis, especially for amyloids.
- Read "STEM Specimen Preparation" below and note any problems you anticipate
- E-mail or telephone (note: best times for telephone are M, W, T, 1-7 pm)
- After discussion, provide a 1-paragraph summary of your goals for the STEM project and a description of the sample(s)
Also please provide any relevant background material.
- Arrange finances (~$400/grid), shipping date (with Beth Lin) and ship specimen by overnight package delivery (e.g. FedEx).
Check with NIH whether your program manager is willing to provide a supplement to cover STEM charges (many are).
- We will prepare grids on the day the specimen(s) arrive and store them under liquid nitrogen until STEM time is available.
We will advise you on estimated turnaround time and send e-mail when images are available on ftp.stem.bnl.gov.
(Note: visits to BNL are welcome. Non-US citizens need prior approval (months) and all visitors must complete a Guest Information Form. Overnight freeze-drying means at least a 2-day visit unless your specimens are sent in advance. Housing is available on-site or in the area. Lab space can be arranged if time-critical preparations are required.)
Last Modified: June 12, 2009
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