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U9B/U11

Ultraviolet Circular Dichroism and Time-Resolved Fluorescence

An Outside User Facility at the NSLS UV-Ring

Contact:
John C. Sutherland
Biology Department, 463
Brookhaven National Laboratory
Upton, NY 11973-5000
tel: (631) 344-3279
fax: (631) 344-3407
jcs@bnl.gov

U9B Group:
John Trunk                (631) 344-3406     trunk@bnl.gov
NSLS U9B Beamline     (631) 344-5509
NSLS U11 Beamline     (631) 344-5511

NSLS U9B Beamline  is designed for circular dichroism (CD/MCD/LD) in the vacuum ultraviolet to about 160 nm using a calcium fluoride photoelastic modulator. Sample absorption spectra are acquired during the same scan [1]. CD of samples in an aqueous environment can be recorded to 162 nm using varaible pathlength sample cells [2]. Sample temperatures can be controlled in the range from -10˚C to + 90˚C. CD experiments can also be performed at 77˚K or < 20˚C.

Fluorescence (excitation spectra, emission spectra, and decay histograms) are recorded by single-photon counting techniques using either the vacuum spectrometer (also used for CD) or a sparate non-vacuum spectrometer. Temporal resolution is about 100 ps [3]. Samples can be in cuvettes, or isolated in matrices of nitrogen or argon at cryogenic temperatures [4]. A recently developed system, the Fluorescence Omnilyzer, permits simultaneous recording of the temporal decay profiles of the entire emission spectrum [5], and its polarization anisotropy [6].

Excellent CD spectra to 130 nm have been recorded using B>Beamline U11, and this limit has been extended to ~110 nm using a LiF modulator.

Both spectrometers are operated by a Windows PC with archival data storage on a Redhat Linux server.

Denise Monteleone and John Trunk
working at U9B beam-line

References

• Kelly L.A., Trunk J.G. and Sutherland J.C.
  Time resolved fluorescence polarization measurements for entire emission spectra with a resistive-anode, single-photon-counting detector: the Fluorescence Omnilyzer.
  Rev. Sci. Instr., 68:2279-2286 (1997). Abstract
• Kelly L.A., Trunk J.G., Polewski  K. and Sutherland, J.C.
  Simultaneous resolution of spectral and temporal properites of UV and visible fluorescence using single-photon counting with a position-sensitive detector.
  Rev. Sci. Instr., 66:496-1498 (1995). Abstract
• Polewski K., Zinger D., Trunk J., Monteleone D.C. and Sutherland J.C.
  Fluorescence of matrix isolated guanine and 7-methylguanine.
  J. Photochem. Photobiol., 24:169-177 (1994). Medline Abstract
• Polewski K., Kramer S.L., Kolber Z.S., Trunk J.G., Monteleone D.C. and Sutherland J.C.
  Time resolved fluorescence using synchrotron radiation excitation: a powered fourth harmonic cavity improves pulse stability.
  Rev. Sci. Instr., 65:2562-2567 (1994). Abstract
• Gray, D.M., Lang D., Kuner E., Vaughan, M. and Sutherland, J.C.
  Thin quartz cell suitable for vacuum ultraviolet absorption and circular dichroism measurements.
  Anal. Biochem., 136:247-250 (1984). Abstract
• Sutherland J.C., Desmond E.J. and Takacs P.Z.
  Versatile spectrometer for experiments using synchrotron radiation at wavelengths greater than 100 nm
  Nucl. Instr. Meth., 172:195-199 (1980).  Abstract

Last Modified: May 6, 2009
Please forward all questions about this site to: Denise Monteleone

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