General Information

Top of Page

Coherent Diffractive Imaging (CDI) for Biological Applications at NSLS-II

May 17-18, 2010

Brookhaven National Laboratory, Bldg. 703, Large Conference Room

Organizing Committee: John Maio (UCLA), Thomas Earnest (LBNL), David Shapiro (BNL), Enju Lima (BNL), Wayne Hendrickson (Columbia University/BNL)

Coherent X-ray Imaging (CXI) is one of the fastest-growing fields in X-ray sciences and the development of CXI methods has been burgeoning at several facilities worldwide. However, a significant challenge the community faces in the United States is gaining sufficient access to dedicated CXI beamlines. The situation will be changed with the advent of NSLS-II which will be one of the brightest synchrotron radiation sources in the world. Through bringing together some of the leading researchers in CXI and potential users, this workshop aims to:

(i) Identify strong scientific cases in biology-related fields for the construction of a dedicated CXI beamline at NSLS-II.

(ii) Identify the best CXI methodologies that can be realized at NSLS-II for pursuing world-class research.

(iii) Identify critical beamline parameters, such as the optimum energy range, the required coherent flux, monochromaticity and the desired spatial resolutions necessary to open up new avenues of research.

 (vi) Identify the best sample preparation and data analysis procedures to facilitate CXI of biological samples at NSLS-II.

Speakers

Thomas Earnest, Lawrence Berkeley National Lab / Xiaojing Huang, Argonne National Laboratory / Enju Lima, Brookhaven National Laboratory / Lee Makowski, Argonne National Laboratory / John Miao, University of California, Los Angeles /Ian McNulty, Argonne National Laboratory / Yoshinori Nishino, Hokkaido University, Japan / Keith Nugent, University of Melbourne / Abbas Ourmazd, University of Wisconsin at Milwaukee / Jeremy Phillips, UCSF / David Shapiro, Brookhaven National Laboratory / Qun Shen, Brookhaven National Laboratory / Changyong Song, SPring-8 / RIKEN / Fuyu Tamanoi, University of California, Los Angeles / Robert Thorne, Cornell University / Gayle Woloschak, Northwestern University /

Agenda

Monday, May 17

8:00 - 8:30     Continental Breakfast

8:30 - 8:45     Wayne Hendrickson (Columbia University) - Welcome, Goals & New Opportunity

Session I: Overview      Chair: Wayne Hendrickson (Columbia University)

8:45 - 9:15
Qun Shen (Brookhaven National Laboratory

NSLS-II Overview and Opportunity for Coherent Diffractive Imaging (Tentative)

9:15 - 9:45 Keith Nugent (University of Melbourne) CDI, Ptychography and Phase-Diversity
9:45 - 10:15 Thomas Earnest (Lawrence Berkeley National Lab) Coherent Diffractive Imaging for Biology and Bio/Nano-Science
10:15 10:35 Coffee Break

Session II:  CDI and Its Biological Applications (I) Chair:  Qun Shen (Brookhaven National Laboratory)

10:35 - 11:05
John Miao (University of California, Los Angeles)

Coherent Diffractive Imaging of Biological Samples

11:05 11:35 Yoshinori Nishino (Hokkaido University)

X-ray Diffraction Microscopy for Cellular Organelles

11:35 - 12:05 David Shapiro (Brookhaven National Lab) TBD
12:05 - 13:30 Lunch

Session III:  Cell and Structural Biology  Chair:  Lisa Miller (Brookhaven National Laboratory)

13:30 14:00
Gayle Woloschak (Northwestern University)

Imaging Applications for Detection of TiO2 Nanoparticles in Mammalian Cells and Tissues

14:00 14:30 Lee Makowski (Argonne National Laboratory) In Situ Imaging of Cellulose Crystals
14:30 15:00 Fuyu Tamanoi (University of California, Los Angeles)

Viewing Yeast Spores in 3D: Future Prospects

15:00 15:30 Jeremy Phillips (University of California, San Francisco) Towards an atomic structure of the Nuclear Pore Complex
15:30 15:50 Coffee Break

Session IV:  CDI and Its Biological Applications (II)     Chair:  Keith Nugent (University of Melbourne)

15:50 - 16:20
Changyong Song (SPring-8 / RIKEN)

Biological 3D imaging with Intense, Coherent X-rays

16:20 16:50 Robert Thorne (Cornell University) Cryopreservation of Biological Samples: From Art to Science
16:50 17:20 Abbas Ourmazd (University of Wisconsin at Milwaukee) Tomography with Random Snapshots of Faint Non-Stationary Objects
17:20 - 18:00 Discussion
18:30 Dinner

Tuesday, May 18

8:00 - 8:30     Continental Breakfast

 Session V: CDI and Its Biological Applications (III)      Chair: Lee Makowski (Argonne National Laboratory)

8:30 - 9:00
Ian McNulty (Argonne National Laboratory) Coherent Diffractive Imaging into the Next Decade
9:00 - 9:30 Enju Lima (Brookhaven National Lab)

Cryogenic X-ray Diffraction Microscopy for Biological Samples (Tentative)

9:30 - 10:00 Xiaojing Huang (Argonne National Lab)

X-ray Diffraction Microscopy on Eukaryotic Cells: Progress and Challenges

10:00 - 10:20 Coffee Break

Session VI: Discussion/Planning/Proposal Development     Chair: Thomas Earnest (Lawrence Berkeley Lab)

10:20 - 13:00 Working lunch will be provided at noon.
 

         Identify strong scientific cases in biology-related fields for the construction of a dedicated CDI beamline at NSLS-II.

          Identify the best CDI methodologies that can be realized at NSLS-II for pursuing world-class research.

          Identify critical beamline specifications: the optimal energy range, beamline optics, monochromaticity and desired spatial resolutions necessary to open up new avenues of research.

          Identify the best sample preparation and data analysis procedures to facilitate CDI of biological samples at NSLS-II.

Administrative Contact:  Lydia Rogers, telephone:  631-344-4746; email: lrogers@bnl.gov

Top of Page

Last Modified: May 2, 2014
Please forward all questions about this site to: BNL Web Services