Protein Extraction and Purification

During this workshop, students will extract and purify jellyfish Green Fluorescent Protein (GFP) by hydrophobic interaction chromatography (HIC). Participants will learn how scientists use BNL's National Synchrotron Light Source to understand protein structure and function.

Program Cost

$450

Contact Information

For more information about this program, contact:

Menzel Smith-Jones
Brookhaven National Laboratory
Bldg.400C – P.O. Box 5000
Upton, NY 11973-5000
631-344-4495 (phone)
631-344-7098 (fax)
slc@bnl.gov

Objectives: The students will learn how scientists genetically engineer bacteria to express proteins of scientific or medical interest; learn how to properly use scientific equipment, including micropipettes and micro-centrifuges; learn how to express the recombinant protein, Green Fluorescent Protein (GFP) in bacterial cultures; understand the principles of Hydrophobic Interaction Chromatography (H.I.C.), used to separate and purify GFP; understand the connection between protein structure and protein function; and learn how scientists use information about protein structures to develop strategies for purifying them from cells.

Audience for This Program

Grades 6th, 7th, and 8th