5th Hands-On Workshop On

Making Single Molecule Fluorescence Lifetime Measurements Simple

Motivation & Plans

The workshop is a 2 1/2-day event which will focus on the latest developments in single molecule spectroscopy and fluorescence lifetime imaging. The topics covered during this workshop include: basic single molecule detection techniques, fluorescence microscopy, Fluorescence Lifetime Imaging (FLIM), Förster Resonance Energy Transfer (FRET) and Fluorescence Correlation Spectroscopy (FCS). The workshop will include lectures as well as hands-on experiments.

This workshop is intended for researchers interested in learning about new optical microscopy techniques / methods and single molecule spectroscopy. It is intended for all levels of expertise, including graduate students, technicians and professional researchers. Basic knowledge about light microscopy is beneficial, but not necessary to attend this workshop. The aim of this workshop is to stimulate discussion in this field and give an introduction to single molecule detection (SMD) and Fluorescence Lifetime Imaging (FLIM).

Since space is limited, please register as soon as possible.

Invited Speakers

• Volker Buschmann (PicoQuant GmbH)
  "Recent Technical Developments in Time-Resolved Microscopy down to the Single Molecule Level"
• Mircea Cotlet (Brookhaven National Laboratory)
  "All in One Protein: FRET, Kindling and Blinking in Single Proteins of HcRed"
• Rainer Erdmann (PicoQuant GmbH)
  "Advanced FRET and FCS Measurements with Laser Scanning Microscopes Based on Time-Resolved Techniques"
• Samantha Fore (PicoQuant Photonics North America)
  "Time-Resolved Single Molecule & Antibunching Studies of the MEH-PPV Conjugated Polymer System"
• Zygmunt "Karol" Gryczynski (University of North Texas)
  "Plasmonic Approach to Study Biological Processes"
• Ahmed Heikal (University of Minnesota Duluth)
  "Single-Molecule Diffusion Studies of MHC Class I Proteins in Fibroblast Cells"
• Joseph R. Lakowicz (University of Baltimore)
  "Single Molecule Detection and Photophysics Using Plasmonic Nanostructures"
• Antoine Van Oijen (Harvard Medical School)
  "Single-Molecule Studies of Multi-Protein Complexes"
• Elisabeth Rhoades (Yale University)
  "Probing the Conformations of Polymorphic Proteins"
• Peter T.C. So (Massachusetts Institute of Technology)
  "Wide-Field Two-Photon Imaging and Microfabrication"
• Haw Yang (Princeton University)
  "Protein Large-Amplitude Conformational Transitions: Dynamics, Mechanics, and Functional Roles"

Call for Papers

The time schedule includes 8 time slots for contributed talks (25+5 minutes). If you are interested in giving a contributed talk, please submit a 100 word abstract no later than March 30, 2010 along with your workshop registration. The best abstracts will be selected for a talk and for all other contributions a poster session will be held on the first evening. Submit your abstract to the following email address SMFWorkshop@bnl.gov.

Hands-on Experiments (preliminary)

• Open frame MicroTime 200: a home built single molecule set-up based on PicoQuant core components.
• Olympus FluoView FV1000, upgraded with FLIM and FCS capabilities.
• TIRFM Olympus IX81 with CW excitation and EMCCD detection in dual-view format (two color detection).
• FluoTime 200: time-resolved fluorescence spectrometer.

Joint Sponsors

Poster Session

There will be a poster session on Monday, April 26, 2010.  If you are interested in presenting a poster, please indicate on the Workshop registration form.

Organizing Committee

• Mircea Cotlet (BNL)
• Rainer Erdmann (PicoQuant, Germany)
• Samantha Fore (PicoQuant North America, US)

Previous Workshops

• 2009 | 2008 | 2007 | 2006

Last Modified: September 1, 2010