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Categories: biotechnology & health, environment

BSA 08-03: Fragmented DNA Genome Signature Tags

BNL Reference Number: BSA 08-03

Patent Status: Non-Provisional filed on October 29, 2007

Summary

Analysis of Duplicated SACO Loci (A) Chromosomal map of a gene-rich region of chromosome10 (bp 55,000,000–65,000,000). (B) Clustering of GSTs in two representative promoter regions. Transcriptional start sites are based on ENSEMBL annotation and are indicated by arrows. Vertical bars represent the number of individual GSTs at a given site. (C) Classification of SACO loci within 2 kb of the start sites of annotated genes (5’), based on known (UCSC genome center) or ENSEMBL full-length mRNA gene categories. (D) “Bidirectional” denotes loci flanked by two transcripts (annotated genes or ECgene transcripts) in a head-to-head arrangement. Only bidirectional transcripts separated by less than 2 kb were considered. “Single” denotes loci within 2 kb of a 5’ start site.

In an adaptation of the genome signature tags technology, this invention enables the analysis of GSTs from fragmented DNA samples. The method is particularly useful in forensic, archeological and historical research as it allows specific identification in situations in which DNA preservation was not possible. In addition, the technology can be used to examine the array of genomic DNA fragments that are specifically bound by DNA-binding proteins and transcription factors.

Description

Describes a method of generating genomic signature tags from poorly preserved and purposefully fragmented DNA samples.

Journal Publication

• Defining the CREB Regulon: A Genome-Wide Analysis of Transcription Factor Regulatory Regions (.pdf)

Have Questions?

For more information about this technology, contact Christine Brakel, (631) 344-7134.