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General Information
Category: biotechnology & health
BSA 94-18: T7 Gene Expression System
BNL Reference Number: BSA 94-18
Patent Status: U.S. Patent Number 5,693,489 was issued on December 2, 1997
Summary
T7 Expression System
The expression of recombinant proteins in bacterial cells under the control of the T7 RNA polymerase promoter is one of the most highly productive means for preparing large quantities of a protein from its cloned gene. Various expression vectors and a variety of host expression cells are available from licensed distributors. Commercial entities must have a license agreement in place with Brookhaven to purchase and use the vectors and host cells.
Description
The T7 RNA polymerase enzyme is without a doubt one of the most active RNA polymerase enzymes ever known. Its use in recombinant protein expression is well documented and widely used. The enzyme is induced from the T7 gene 1 sequence resident in host expression cells. The enzyme is highly specific for its own promoter and generates very large amounts of mRNA from genes that are controlled by the promoter. The result is most generally very large amounts of the desired target protein.
Benefits
The use of this invention is enabled by the availability of host cells and vectors. Please contact Brookhaven National Laboratory for information for licensing the use of the invention.
Applications and Industries
Research and industry manufacturers of recombinant proteins have made use of this expression system and for the study of proteins, this system has been shown to be superior to many alternative expression systems.
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Have Questions?
For more information about this technology, contact Christine Brakel, (631) 344-7134.
