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Joseph S. Wall


Research Interests

Mass mapping of unstained biological molecules with the scanning transmission electron microscope (STEM), particularly assemblies of complexes from subunits of known size and shape. Examples include: Alzheimer's filaments, viral capsids, annelid hemoglobins, hemocyanins, proteases, chaperonins, microtubule proteins, prions and various nucleic acid-protein complexes. Another research area is instrument development involving design and construction of an instrument for low-temperture, energy loss spectroscopy, and elemental mapping at low dose. This is being used to map phosphorus in nucleic acid-protein complexes, phosphorylated proteins and phospholipid structures.

He also is director of the Scanning Transmission Electron Microscope STEM Facility which currently hosts over 50 user projects per year from around the world. These projects focus on use of the STEM for mass measurements of single molecules, or of gold cluster labeling for identifying molecular sites. Wall represents BNL (along with Y. Zhu) on DOE's TEAM Project (Transmission Electron Aberration-corrected Electron Microscope) which seeks to develop instruments with 0.5Å resolution. He is active in the CFN (Center for Functional Nanomaterials) in demonstrating the capabilities of the state-of-the-art aberration-corrected instruments recently installed there.

Selected Publications

  • Hodgkinson, J. L., Horsley, A., Stabat, D., Simon, M., Johnson, S., da Fonseca, P. C., Morris, E. P., Wall J.S., Lea, S. M., and Blocker, A. J.
    Three-dimensional reconstruction of the Shigella T3SS transmembrane regions reveals 12-fold symmetry and novel features throughout.
    Nature Structural and Molecular Biology 16(5): 477-485 (May, 2009). (News & Views: Waksman, G. Going round in circles: The structural biology of type III secretion systems.
    Nature Structural & Molecular Biology, 16(5):459-460 (May, 2009).  PubMed
  • Inada, H., Wu, L., Wall J., Su, D., and Zhu, Y.
    Performance and image analysis of the aberration corrected Hitachi HD-2700C STEM.
    J. Electron. Microsc. (Tokyo), 58(3):111-122. Epub (2009).  PubMed
  • Wall J.S., Simon, M. N., and Hainfeld, J. F.
    History of the STEM at BNL. Advances in Imaging & Electron Physics, P. W. Hawkes, Editor, Vol. 158, Elsevier Academic Press, U.K. (accepted).
  • Kendall A., McDonald M., Bian W., Bowles T., Baumgarten S.C., Shi J., Stewart P.L., Bullitt E., Gore D., Irving T.C., Havens W.M., Ghabrial S.A., Wall J.S. and Stubbs G.
    Structure of flexible filamentous plant viruses.
    Journal of Virology, 82(19):9546-9554 (2008).  PubMed
  • Volkov V.V., Wall J. and Zhu Y.
    Position-sensitive diffractive imaging in STEM by an automated chaining diffraction algorithm.
    Ultramicroscopy, 108(8):741-749 (2008). Cover: Figure 4 appears on the cover.   PubMed
  • Wall J.S., Simon M.N., Lin B.Y. and Vinogradov S.N.
    Mass mapping of large globin complexes by scanning transmission electron microscopy.
    Methods in Enzymology, Vol. 436: Globins and Other Nitric Oxide-Reactive Proteins, Part A, Chapter 27, pp. 487-501, Elsevier, Inc. (2008).  PubMed
  • Deptuch G., Besson A., Rehak P., Szelezniak M., Wall J., Winter M., Zhu Y.
    Direct Electron Imaging in Electron Microscopy with Monolithic Active Pixel Sensors.
    Ultramicroscopy, 107(8):674-684 (2007).  PubMed
  • Jiang Z.G., Simon M.N., Wall J.S., and McKnight C.J.
    Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B.
    Biophysical J., 92(11):4097-4108 (2007).  PubMed
  • Ohi M.D., Feoktistova A., Ren L., Yip C., Cheng Y., Chen J.S., Yoon H.J., Wall J.S., Huang Z., Penczek P.A., Gould K.L., and Walz T.
    Structural organization of the anaphase-promoting complex bound to the mitotic activator slp1.
    Molecular Cell, 28(5):871-85 (2007).  PubMed
  • Ohi M.D., Ren L., Wall J.S., Gould K.L., and Walz T.
    Structural characterization of the fission yeast U5.U2/U6 spliceosome complex.
    Proc National Acad of Sci USA, 104(9):3195-3200 (2007).  PubMed
  • Sen A., Baxa U., Simon M.N., Wall J.S., Sabate R., Saupe S.J., and Steven A.C.
    Mass analysis by scanning transmission electron microscopy and electron diffraction validate predictions of the stacked β-solenoid model of HET-s prion fibrils.
    J of Bio Chem ., 282(8):5545-50 (2007).  PubMed
  • Spano A.J., Chen F.S., Goodman B.E., Sabat A.E., Simon M.N., Wall J.S., Correia J.J., McIvor W., Newcomb W.W., Brown J.C., Schnur J.M., and Lebedev N.
    In vitro assembly of a prohead-like structure of the Rhodobacter capsulatus gene transfer agent.
    Virology, 364(1):95-102 (2007).  PubMed
  • Diaz-Avalos R., King C.Y., Wall J.S., Simon M.N., and Caspar D.L.
    Strain-specific morphologies of yeast prion amyloid fibrils.
    Proc Natl Acad Sci USA, 102(29):10165-70 (2005).   PubMed   Full Text
  • Ksiezak-Reding H., and Wall J.S.
    Characterization of paired helical filaments by scanning transmission electron microscopy.
    Micros Res Tech., 67(3-4):126-140 (2005).   PubMed
  • Lashuel H.A., and Wall J.S.
    Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis.
    in: Methods in Molecular Biology Vol. 299: Amyloid Proteins: Methods and Protocols, E. M. Sigurdsson, Editor, Chapter 7, pp. 81-102, Humana Press, Inc., Totowa, NJ (2005).   PubMed
  • Ding S.-Y., Rumbles G., Jones M., Tucker M.P., Nedeljkovic J., Simon M.N., Wall J.S., Himmel M.E.
    Bioconjugation of (CdSe)ZnS quantum dots using a genetically engineered multiple polyhistidine tagged cohesin/dockerin protein polymer.
    Macromol Materials Eng. 289:622-628 (2004).
  • Mosesson M.W., DiOrio J.P., Hernandez I., Hainfeld J.F., Wall J.S., and Grieninger G.
    The ultrastructure of fibrinogen-420 and the fibrin-420 clot.
    Biophys Chem., 112: 209-214 (2004).   PubMed
  • Baxa U., Taylor K.L., Wall J.S., Simon M.N., Cheng N., Wickner R.B., and Steven A.C.
    Architecture of Ure2p prion filaments: The N-terminal domains form a central core fiber.
    J Biol Chem., 278(44):43717-27 (2003).   PubMed   Full Text
  • Chen W., De Geronimo G., Li Z., O'Connor P., Radeka V., Rehak P., Smith G.C., Wall J.S., Yu B.
    High resistivity silicon active pixel sensors for recording data
    from STEM Nuclear Instruments & Methods in Physics research section A-accelerators Spectrometers Detectors and Associated equipment, 512:368-377 (2003).
  • Ding S.-Y., Jones M., Tucker M.P., Nedeljkovic J.M., Wall J., Simon M.N., Rumbles G., and Himmel M.E.
    Quantum Dot Molecules Assembled with Genetically Engineered Proteins.
    NanoLetters, 3:1581-1585 (2003).
  • Ding S.-Y., Rumbles G., Jones M., Tucker M.P., Nedeljkovic J., Wall J., and Himmel M.E.
    Quantum Dots Stabilized By Genetically Engineered Proteins.
    Mat Res Soc Symp Proc., 774:165-170 (2003).
  • Lashuel H.A., Hartley D.M., Petre B.M., Wall J.S., Simon M.N., Walz T., and Lansbury P.T.
    Mixtures of wild-type and a pathogenic (E22G) form of Abeta40 in vitro accumulate protofibrils, including amyloid pores.
    J Mol Biol., 332:795-808 (2003).   PubMed
  • Mosesson M.W., Siebenlist K.R., Hernandez I., Wall J.S. and Hainfeld J.F.
    Fibrinogen assembly and crosslinking on a fibrin fragment E template.
    Thromb Haemost., 87:651-8 (2002).   PubMed
  • Watts N.R., Jones L.N., Cheng N., Wall J.S., Parry D.A. and Steven A.C.
    Cryo-electron microscopy of trichocyte (hard alpha-keratin) intermediate filaments reveals a low-density core.
    J Struct Biol., 137:109-18 (2002).   PubMed
  • King M.E., Ghoshal N., Wall J.S., Binder L.I., and Ksiezak-Reding H.
    Structural analysis of Pick's disease-derived and in vitro-assembled tau filaments.
    Am J Pathol., 158:1481-90 (2001).   PubMed   Full Text
  • Parker S.D., Wall J.S. and HunterF.
    Analysis of Mason-Pfizer monkey virus Gag particles by scanning transmission electron microscopy.
    J Virol., 75: 9543-8 (2001).   PubMed   Full Text
  • Wall J.S. and Simon M.N.
    Scanning transmission electron microscopy of DNA-protein complexes.
    Methods Mol Biol., 148:589-601 (2001).   PubMed
  • Kuchumov A.R., Taveau J.C., Lamy J.N., Wall J.S., Weber R.E., and Vinogradov S.N.
    The role of linkers in the reassembly of the 3.6 MDa hexagonal bilayer hemoglobin from Lumbricus terrestris.
    J Mol Biol., 289:1361-1374 (1999).   PubMed
  • Wall J.S.
    Visualizing "greengold" clusters in the STEM.
    J Struct Biol., 27:161-168 (1999).   PubMed
  • Ksiezak-Reding H., Yang G., Simon M., and Wall J.S.
    Assembled tau filaments differ from native paired helical filaments as determined by scanning transmission electron microscopy (STEM).
    Brain Res., 814:86-98 (1998).   PubMed
  • Mosesson M.W., Siebenlist K.R., Meh D.A., Wall J.S. and Hainfeld J.F.
    The location of the carboxy-terminal region of gamma chains in fibrinogen and fibrin D domains.
    Proc Natl Acad Sci USA, 95:10511-10516 (1998).   PubMed   Full Text
  • Wall J.S., Hainfeld J.F. and Simon M.N.
    Scanning transmission electron microscopy of nuclear structures.
    In: Nuclear Strcture and Function, M. Berrios (ed.) Methods in Cell Biology, vol. 53, San Diego: Academic Press, pp 139-164 (1998).   PubMed
  • Thomas D., Schultz P., Steven A.C., and Wall J.S.
    Mass analysis of biological macromolecular complexes by STEM.
    Biol Cell, 80:181-192 (1994).   PubMed
  • Tumminia S.J., Hellmann W., Wall J.S. and Boublik M.
    Visualization of the protein-nucleic acid interactions involved in the in vitro assembly of the Escherichia coli 50S ribosomal subunit.
    J Mol Biol., 235:1239-1250 (1994).   PubMed
  • Mosesson M.W., Di Orio J.P., Siebenlist K.R., Wall J.S. and Hainfeld J.F.
    Evidence for a second type of fibril branch point in fibrin polymer networks,the trimolecular junction.
    Blood, 82:1517-1521 (1993).   PubMed
  • Wall J.S. and Hainfeld J.F.
    Mass mapping with the Scanning Transmission Electron Microscope.
    Ann Rev Biophys Chem., 15:355-376 (1986).   PubMed