I am primarily interested in determining how ionizing radiation affects
cells of the human central nervous system. Radiation from high energy,
charged ions found outside of the earth's protective magnetosphere have
great potential to cause acute and chronic health problems for astronauts
during and after space missions. To assess human risk and determine the
molecular mechanisms of radiation induced damage, we use a model in vitro
system consisting of human neuronal progenitor cells and their terminally
differentiated, post-mitotic derivatives (neurons). Experiments are
performed following irradiation of these cells to measure effects such as
the inductions of apoptosis and necrosis, changes in gene/protein
expression and perturbations of the cell cycle. In addition,
pharmacological compounds are tested in an effort to counteract the toxic
effects of radiation exposure.
- Sharma, P., Guida, P. and Grabham, P. (2014) Effects of Fe
particle irradiation on human endothelial barrier structure and
function. Life Sciences in Space Research 2: 29-37
- Jacobus J.A., Duda C.G., Coleman M.C., Martin S.M., Mapuskar K., Mao G., Smith B.J.,
Aykin-Burns N., Guida P., Gius D., Domann F.E., Knudson C.M., and Spitz D.R.
Low-dose radiation-induced enhancement of thymic lymphomagenesis in Lck-Bax mice
is dependent on LET and gender.
Radiation Research, 180(2):156-165 (2013).
Loria, R., Beckman, M., Contaifer, D., Tamariz, F., Gibb, D., Thompson, L. and Guida, P.
Beta androstenediol mitigates the damage of 1 GeV/n Fe ion particle radiation to the hematopoietic system.
Cancer Biotherapy & Radiopharmaceuticals, 26(4):453-459 (2011).
- Ponomarev A.L., Sundaresan A., Vazquez M.E., Guida P., Kim A. and Cucinotta F.A.
A model of the effects of heavy ion radiation on human tissue.
Advances in Space Research, 47(1):37-48 (2011).
Yu, X., Wang, H., Liu, S., Zhang, X., Guida, P., Hu, B. and Wang, Y.
A small peptide mimicking the key domain of MEPE/OF45 interacting with CHK1 protects human cells from radiation-induced killing.
Cell Cycle, 9(10):1-5 (2010).
- Roy D., Guida P., Zhou G., Echiburu-Chau C. and Calaf G.M.
Gene expression profiling of breast cells induced by X-rays and heavy ions.
International Journal of Molecular Medcine, 21(5):627-636 (2008).
- Guida P. and Vazquez M.E.
Cytotoxic and cell cycle effects in human neuronal progenitor cells exposed to 1 GeV/n Fe ions.
Advances in Space Research, 39(6):1004-1010 (2007).
- Guida P., Vazquez M.E. and Otto S.
Cytotoxic Effects of low- and high-LET
radiation on human neuronal progenitor cells: induction of apoptosis and
TP53 gene expression.
Radiation Research, 164(4):545-551 (2005).
- Sutherland B.M., Bennett P.V., Cintron N.S., Guida P. and Laval J.
Low levels of endogeneous oxidative damage cluster levels in unirradiated
viral and human DNAs.
Free Radical Biology and Medicine, 35(5):495-503 (2003).
- Jiang H., Karnezis A.N., Tao M., Guida P.M. and Zhu L.
pRB and p107 have distinct effects when expressed in pRB-deficient tumor cells at physiologically relevant levels.
Oncogene, 19(34):3878-3887 (2000).
- Guida P. and Zhu L.
DP1 phosphorylation in multimeric complexes: weaker
interaction with cyclin A through the E2F1 cyclin A binding domain leads
to more efficient phosphorylation than stronger interaction through the
p107 cyclin A binding domain.
Biochemical and Biophysical Research Communications, 258(3):596-604 (1999).
- Rao G., Alland L., Guida P., Schreiber-Agus N., Chen K., Chin L., Rochelle
J.M., Seldin M.F., Skoultchi A.I. and DePinho R.A.
Mouse Sin3A interacts with and can functionally substitute for the amino-terminal
repression of the Myc antagonist Mxi1.
Oncogene, 12(5):1165-1172 (1996).
- Schreiber-Agus N., Chin L., Chen K., Torres R., Rao G., Guida P., Skoultchi A.I.
and DePinho R.A.
An amino-terminal domain of Mxi1 mediates anti-Myc
oncogenic activity and interacts with a homolog of the yeast
transcriptional repressor SIN3.
Cell, 80(5):777-786 (1995).