Biological, Environmental, & Climate Sciences (BECS) Department Seminar
"Molecular Mechanism of the Assembly of PKD1/TRPP2 Receptor-Ion Channel Complex, the Cellular Sensor in Autosomal Dominant Polycystic Kidney Disease"
Presented by Yong Yu, Department of Biological Sciences, St. John's University, Queens, NY
Friday, August 28, 2015, 11:00 am — John Dunn Seminar Room, Bldg. 463
Mutations in TRPP2 and PKD1 account for almost all clinically identified cases of autosomal dominant polycystic kidney disease (ADPKD), one of the most common human genetic diseases. TRPP2 and PKD1 form a receptor-ion channel complex on primary cilia and couple extracellular stimuli to cellular responses in renal cells. We found that this complex contains three TRPP2 subunits and one PKD1 subunit, and this 3:1 stoichiometry is determined by a coiled-coil domain on the C-terminus of TRPP2. Our data also suggested that PKD proteins may be involved in channel pore-forming, in addition to signal reception. Furthermore, by generating a gain-of-function TRPP2 mutant, we investigated the functional property of TRPP2, which was largely unknown due to the lack of the knowledge on the activation mechanism of this ion channel.
Hosted by: Chang-Jun Liu
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