NSLS-II Colloquium Series
"Biophysical Studies of an RNA Virus particle and its Maturation: Insights into an Elegantly Programmed Nano-machine"
Presented by John E. (Jack) Johnson, Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Thursday, October 11, 2018, 4:00 pm — Large Seminar Room, Bldg. 510
Nudaurelia Capensis ? Virus (N?V) is a eukaryotic, quasi-equivalent, RNA virus, with a T=4 surface lattice, where maturation is dramatic (a change in particle size of 100Å) and is novel in that it can be investigated in vitro. Here we use X-ray crystallography, biochemistry, Small Angle X-ray Scattering, and electron cryo-microscopy and image reconstruction (CryoEM), to characterize maturation intermediates, an associated auto-catalytic cleavage, the kinetics of morphological change and to demonstrate that regions of N?V subunit folding are maturation-dependent and occur at rates determined by their quasi-equivalent position in the capsid. Matsui, T., Lander, G. C., Khayat, R., and Johnson, J. E. 2010. Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus. Proc Natl Acad Sci U S A 107:14111-5. Veesler, D., and Johnson, J.E. 2012. Virus Maturation. Annual review of biophysics 41:473-496. Doerschuk, P. C., Gong, Y., Xu, N., Domitrovic, T., and Johnson, J. E. 2016. Virus particle dynamics derived from CryoEM studies. Curr Opin Virol 18:57-63.
Hosted by: John Hill
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