NSLS-II Colloquium Series

"Biophysical Studies of an RNA Virus particle and its Maturation: Insights into an Elegantly Programmed Nano-machine"

Presented by John E. (Jack) Johnson, Department of Integrative Structural and Computational Biology, The Scripps Research Institute

Thursday, October 11, 2018, 4:00 pm — Large Seminar Room, Bldg. 510

Nudaurelia Capensis ? Virus (N?V) is a eukaryotic, quasi-equivalent, RNA virus, with a T=4 surface lattice, where maturation is dramatic (a change in particle size of 100Å) and is novel in that it can be investigated in vitro. Here we use X-ray crystallography, biochemistry, Small Angle X-ray Scattering, and electron cryo-microscopy and image reconstruction (CryoEM), to characterize maturation intermediates, an associated auto-catalytic cleavage, the kinetics of morphological change and to demonstrate that regions of N?V subunit folding are maturation-dependent and occur at rates determined by their quasi-equivalent position in the capsid. Matsui, T., Lander, G. C., Khayat, R., and Johnson, J. E. 2010. Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus. Proc Natl Acad Sci U S A 107:14111-5. Veesler, D., and Johnson, J.E. 2012. Virus Maturation. Annual review of biophysics 41:473-496. Doerschuk, P. C., Gong, Y., Xu, N., Domitrovic, T., and Johnson, J. E. 2016. Virus particle dynamics derived from CryoEM studies. Curr Opin Virol 18:57-63.

Hosted by: John Hill

14316  |  INT/EXT  |  Events Calendar


Not all computers/devices will add this event to your calendar automatically.

A calendar event file named "calendar.ics" will be placed in your downloads location. Depending on how your device/computer is configured, you may have to locate this file and double click on it to add the event to your calendar.

Event dates, times, and locations are subject to change. Event details will not be updated automatically once you add this event to your own calendar. Check the Lab's Events Calendar to ensure that you have the latest event information.