Wednesday, June 28, 2023, 1:30 pm — Videoconference / Virtual Event (see link below)
Phototransformable fluorescent proteins (PTFPs) are widely used in many advanced fluorescence microscopy methods, notably in the super-resolution field. A plethora of PTFPs have been engineered in the last years, which display a variety of photoactivation, photoconversion, photoswitching, photoblinking and photobleaching properties that can be used at advantage in many super-resolution schemes, but that are also at the origin of significant complications and artifacts, making these fascinating genetically encoded labels still far from ideal.
In this talk, I will review how (kinetic) X-ray crystallography, optical spectroscopy, multidimensional NMR and molecular dynamics simulations can be combined with single-molecule investigations to reveal phototransformation mechanisms of PTFPs at the near-atomic level.
Hosted by: Vivian Stojanoff
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