Wednesday, April 23, 2025, 1:30 pm — Videoconference / Virtual Event (see link below)
Enzyme catalysis is essential for life and is a central phenomenon in biochemistry. The advent of time-resolved serial crystallography, initially enabled by X-ray free electron lasers (XFELs) and now expanding to synchrotron X-ray sources, allows enzyme catalysis to be observed in real time, in near-physiological conditions, and at atomic resolution. I will describe our work using mix-and-inject serial crystallography (MISC) to observe catalysis by isocyanide hydratase (ICH). MISC allowed us to observe formation of an unusual thioimidate intermediate and to watch ICH's conformational dynamics respond to changes in active site ionization during catalysis. We also used an engineered ICH mutant to enrich for rare conformations during catalysis, permitting a clearer view of later steps in the reaction. ICH exemplifies a class of enzymes whose non-equilibrium dynamics are gated by changes in active site electrostatics, which is a potentially common enzymological phenomenon.
Hosted by: Vivian Stojanoff
Pre-registration required
Join Videoconference More Information
21224 | INT/EXT | Events Calendar
Not all computers/devices will add this event to your calendar automatically.
A calendar event file named "calendar.ics" will be placed in your downloads location. Depending on how your device/computer is configured, you may have to locate this file and double click on it to add the event to your calendar.
Event dates, times, and locations are subject to change. Event details will not be updated automatically once you add this event to your own calendar. Check the Lab's Events Calendar to ensure that you have the latest event information.