Center for Functional Nanomaterials Seminar

"Single-Molecule Confocal Microscopy for Studying Conformational Changes of Light-Harvesting Complex in the Photosynthetic Membrane"

Presented by Duohai Pan, Beckman Institute at UIUC

Tuesday, February 20, 2007, 10:00 am — Rm. 300, Bldg. 555

Single molecule spectroscopy and confocal optical imaging methods have been rapidly become an important new set of tools for probing the individual nanoscale behavior of molecules in complex local environments. In this talk, I will first present the multi-channel confocal fluorescence spectroscopy and imaging technique which was developed recently. This system allows us to study the fluorescence intensity, lifetime, fluorescence resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) at single molecule level. Second, I will present a study of light-harvesting complexes at room temperature with this system. The fluctuating inter-molecule protein energy transfer in bacterial photosynthetic membranes was observed. The results suggest that there are two states (dynamic coupled and non-coupled states) of the light-harvesting protein assemblies in photosynthetic membranes.

Hosted by: Oleg Gang

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