Thursday, February 22, 2007, 10:00 am — Conference Room, Bldg. 480
Single molecule observations of long-lived molecular complexes tethered to a surface or trapped bead are now becoming a commonplace tool for molecular biology and biophysics. In contrast, the study of more commonly-occurring complexes that interact transiently is complicated by the need for containment and mixing of the components. I will review the advantages and pitfalls of single molecule optical measurement as currently implemented, and then present a new method for biomolecular confinement, manipulation and interrogation that permits the study of transient complexes. In this technique, optically trapped water droplets are used as attoliter reactor vessels for studying individual nano-biological interactions. I will discuss the application of this method to the study of RNA/protein complexes such as the R1-R2/Rom complex of ColE1 plasmid or the DIS/Ncp7 complex in HIV. This includes the use of single molecular-pair fluorescence resonance energy transfer and single molecule polarization anisotropy to elucidate the structure and dynamics of these complexes and their components.
Hosted by: Oleg Gang
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