Biology Department Seminar

"Spectroscopic analysis of protein-ligand interactions: Fluorescent proteins and excited state proton transfer"

Presented by Deborah Stoner-Ma, Stony Brook University

Friday, October 16, 2009, 1:00 pm — John Dunn Seminar Room, Bldg. 463

Members of the green fluorescent protein (GFP) family, vital tools for the imaging of proteins and their interactions, display interesting photophysical properties which can be explored with the use of spectroscopy. A variety of such methods have been used to examine the process of excited state proton transfer (ESPT) in several GFP variants. Our results, highlighted in this talk, indicate a strong propensity for ESPT in these proteins, provided the existence of a proton wire leading to a stable acceptor. In addition, recent work utilizing Raman spectroscopy to study protein-ligand interactions will be presented.


Hosted by: Allen Orville

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