Friday, April 29, 2011, 11:00 am — John Dunn Seminar Room, Bldg. 463
Engineered human adenoviruses are used as therapeutic tools. Understanding the determinants of capsid assembly and stability would help to design more efficient vectors. The last stage in adenovirus assembly is proteolytic maturation. A thermosensitive mutant, Ad2 ts1, does not package the viral protease and produces capsids containing unprocessed protein precursors, providing a good model to study the behavior of immature virions. We have analyzed in detail the disruption of mature and immature adenovirus under different sources of stress. Differential scanning calorimetry, extrinsic fluorescence, electron and atomic force microscopy are used to monitor the process. Ad2 ts1 displays higher stability than wildtype in all disruption assays. That is, the immature adenovirus virion is not a transient, unstable intermediate, but rather a hyperstable particle. Different kinds of stress result in different capsid burst patterns. We use partially disrupted immature viruses as platforms to investigate the viral protease activity within the virion.
Hosted by: Wally Mangel
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