Wednesday, May 9, 2012, 11:00 am — Conference Room C, Bldg.725
Differential Scanning Fluorimetry on Rotor Gene Q and QIAgility: Fast Screening Methods for Protein Analysis
The method we will discussing is Differential Scanning Fluorimetry and below are some examples of where DSF can provide helpful information when studying proteins
> 1. Generate stability data on proteins with different additives and buffers.
> 2. Determine different protein interactions
> 3. Test efficient Ligand binding.
> 4. Run mutant screenings from protein to protein.
The Rotor Gene provides extremely high uniformity; due to even temperature distribution they can detect small. fluorescence differences between samples or states, more efficiently than any block based system.
In addition the Rotor Gene Q is a real time cycler which can also perform:
Quantitation
Melt Curve Analysis
Two Standard Curve method
Delta Delta CT method
Comparative Quantitation
HRM
End Point PCR
Allelic Discrimination
Scatter Plots
Hosted by: Vivian Stojanoff
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