Workshop 2: Cryo-ET Sample Preparation, Screening, Data Collection and Analysis (In-person)

About LBMS

The mission of the Laboratory for BioMolecular Structure (LBMS) is to accelerate our fundamental understanding about the building blocks of all living organisms and their function. By gaining the fundamental knowledge, LBMS aims to foster faster developments in biotechnology and medicine to meet the Nation's needs in biofuels and healthcare. LBMS fulfills its mission by offering expertise and access to highly advanced cryo-electron microscopic capabilities to the greater research community.

Purpose of Workshop

This workshop will focus on cryo-Electron Tomography, covering various aspects such as sample preparation (plunge freezing and cryo-FIB milling), data collection employing high-end EMs (e.g., Krios), and data analysis using LBMS GPU computers with modern software. To optimize interactions and hands-on experience, this workshop will be limited to 16 participants, selected based on their application materials.

Workshop Committee

• Liguo Wang (LBMS, Brookhaven National Laboratory)
  
• Jun Liu (Dept. of Microbial Pathogenesis, Yale University)
  
• Guobin Hu (LBMS, Brookhaven National Laboratory
  
• Jake Kaminsky (LBMS, Brookhaven National Laboratory)
  
• Qun Liu (Biology Dept., Brookhaven National Laboratory)
  
• Yong Xiong (Dept. of Molecular Biophysics and Biochemistry, Yale University)
  
• Dongyan Tan (Dept. of Pharmacological Sciences, Stony Brook University)
  
• Sean McSweeney (NSLS-II, Brookhaven National Laboratory)

Workshop Sponsors

Sponsor support provided by Thermo Fisher Scientific.