General Lab Information

Jean Jakoncic

AMX Lead Beamline Scientist, Structural Biology Program, National Synchrotron Light Source II

Jean Jakoncic

Brookhaven National Laboratory

National Synchrotron Light Source II
Bldg. 745
P.O. Box 5000
Upton, NY 11973-5000

(631) 344-3930
jjakoncic@bnl.gov

Jean Jakoncic is the lead beamline scientist at the highly Automated Macromolecular Crystallography beamline (AMX) NSLS-II. At AMX, we currently focus the beam to an area of 7x5 μm 2 with a measured flux of 4.2 10 12 ph.s -1 at the sample position (13.5 keV). Users routinely collect data from crystals as small as 5 microns in one direction. These beam characteristics, together with the high framing rate detector available to users (Eiger 9M running at up to 238 Hz), have enabled data collection from crystals of the most challenging projects. For some projects, data must be collected from dozens to hundreds to even thousands of micro-crystals for solving the 3D crystal structure.

I’m a crystallographer with extensive expertise in all fields involved in MX encompassing crystallization, data collection, structure solution, molecular structure refinement, structural interpretation, X-ray instrumentation, automation, X-ray detector, X-ray methods, sample visualization, high-energy data collection, low-energy data collection, beamline construction as well as high performance computing deployment to support highly parallel computing for near instant feedback (required for a large fraction of the data collected at our 2 MX beamlines at the NSLS-II, AMX & FMX) and for advanced data processing for multiple to serial crystals data collection across multiple visits requiring hierarchical cluster analysis to sort these many data sets into fewer but useful complete data sets enabling either structure solution otherwise impossible or accessing valuable information about protein dynamics.

Additionally, with colleagues and collaborators, we are tackling multiple crystals (across multiple visits) data collection and data processing using dedicated pipelines. Considering that the instrument/computing I'm working on will enable automated structure solution starting from crystals, will deal with multiple crystals data collection and my extensive experience in collecting data and solving structure from challenging projects, I believe that we will achieve, when required, 1000 screened samples per day, a necessary target to enable structure solution for the most challenging projects and for achieving dynamical states of flexible proteins.

Expertise | Education


Expertise

Structural Biology

X-ray crystallography

Automation

Crystallization

Structure solution: including experimental phasing techniques

Data collection from 100s to 1000s of samples to study protein dynamics

Anomalous scattering

High energy data colleciton

Beamline design/contruction and operation

Experimental end-station design/construction amd operation

Instrumentation, sample automation

HPC to support real time feedback using highly parallel applications

Education

2007     Ph.D.    Structural and Molecular Physical Chemistry, University Joseph Fourier, Grenoble, France                                   

2002     M.S.     Structural and Molecular Physical Chemistry, University Joseph Fourier, Grenoble, France.

Jean Jakoncic

Brookhaven National Laboratory

National Synchrotron Light Source II
Bldg. 745
P.O. Box 5000
Upton, NY 11973-5000

(631) 344-3930
jjakoncic@bnl.gov

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