Liguo Wang

Brookhaven National Laboratory

National Synchrotron Light Source II
Bldg. 745, Room 120
P.O. Box 5000
Upton, NY 11973-5000

(631) 344-7011
lwang1@bnl.gov

Expertise | Education | Appointments | Publications

Expertise

Cryo-Electron Microscopy
Membrane protein structure
Voltage-gated ion channels
Image processing

Education

MBA  — Stony Brook University, Stony Brook, NY, USA, 2023
Ph.D. — Cornell University, Ithaca, NY, USA, 2003
B.S.   — Tsinghua University, Beijing, China, 1994

Professional Appointments

2019-present.   Director of Scientific Operations
                            Laboratory for BioMolecular Structure,
                            Brookhaven National Laboratory, Upton, NY
2011-2019         Assistant Professor of Biological Structure
                            Adjunct Assistant Professor of Chemical Engineering    
                            University of Washington, Seattle, WA
2003-2010         Postdoctoral Associate with Dr. Fred J. Sigworth    
                            Cellular and Molecular Physiology,
                            Yale University, New Haven, CT

Selected Publications

• Zuo H, Park J, Frangaj A, et al (2024) Promiscuous G-protein activation by the calcium-sensing receptor. Nature 629:481–488. https://doi.org/10.1038/s41586-024-07331-1
• Yang X, Wang L, Maxson J, et al (2024) Towards Construction of a Novel Nanometer-Resolution MeV-STEM for Imaging Thick Frozen Biological Samples. Photonics 11:252. https://doi.org/10.3390/photonics11030252
• Wang L, Zimanyi CM (2024) Cryo-EM sample preparation for high-resolution structure studies. Acta Crystallographica Section F Structural Biology Communications 80:74–81. https://doi.org/10.1107/s2053230x24002553
• Wang L, Yang X (2024) Monte Carlo Simulation of Electron Interactions in an MeV-STEM for Thick Frozen Biological Sample Imaging. Applied Sciences 14:1888. https://doi.org/10.3390/app14051888
• Gyawali R, Dhakal A, Wang L, Cheng J (2024) CryoSegNet: accurate cryo-EM protein particle picking by integrating the foundational AI image segmentation model and attention-gated U-Net. Briefings in Bioinformatics 25:. https://doi.org/10.1093/bib/bbae282
• Dhakal A, Gyawali R, Wang L, Cheng J (2023) A large expert-curated cryo-EM image dataset for machine learning protein particle picking. Scientific Data 10:. https://doi.org/10.1038/s41597-023-02280-2
• Tonggu L, Wang L (2022) Structure of the Human BK Ion Channel in Lipid Environment. Membranes 12:758. https://doi.org/10.3390/membranes12080758
• Tonggu L, Wang L (2020) Cryo-EM sample preparation method for extremely low concentration liposomes. Ultramicroscopy 208:112849. https://doi.org/10.1016/j.ultramic.2019.112849
• Wisedchaisri G, Tonggu L, McCord E, et al (2019) Resting-State Structure and Gating Mechanism of a Voltage-Gated Sodium Channel. Cell 178:993-1003.e12. https://doi.org/10.1016/j.cell.2019.06.031
• Li M, Tonggu L, Zhan X, et al (2016) Cryo-EM Visualization of Nanobubbles in Aqueous Solutions. Langmuir 32:11111–11115. https://doi.org/10.1021/acs.langmuir.6b00261
• Li M, Tonggu L, Tang L, Wang L (2015) Effects of N-glycosylation on hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Biochemical Journal 466:77–84. https://doi.org/10.1042/bj20140692
• Wang L (2012) Measurements and Implications of the Membrane Dipole Potential. Annual Review of Biochemistry 81:615–635. https://doi.org/10.1146/annurev-biochem-070110-123033
• Wang L, Sigworth FJ (2010) Liposomes on a Streptavidin Crystal: A System to Study Membrane Proteins by Cryo-EM. Cryo-EM Part A Sample Preparation and Data Collection 147–164. https://doi.org/10.1016/s0076-6879(10)81007-9
• Wang L, Sigworth FJ (2009) Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 461:292–295. https://doi.org/10.1038/nature08291
• Wang L, Bose PS, Sigworth FJ (2006) Using cryo-EM to measure the dipole potential of a lipid membrane. Proceedings of the National Academy of Sciences 103:18528–18533. https://doi.org/10.1073/pnas.0608714103