General Lab Information

Beamlines: Structural Biology Program

About 17-ID-1 AMX Beamline

The beamline offers ultrabright (>4 1012 ph.s-1) x-rays delivered by a canted in-vacuum undulator source (IVU21) with x-ray energies ranging from 5keV to 18keV and a beam size of 7*5 μm2.

The beamline has an adaptable optical system consisting of white beam slits, a double crystal Si(111) monochromator with horizontal theta-axis, tandem flat beam deflecting silicon mirrors (Pd and Si lanes) and Kirkpatrick-Baez focusing silica mirrors, which are Pd coated and able to bent adaptively using 16 piezo actuators.

Each optical element is preceded by slits and its transmitted beam is monitored by beam position monitors or retractable screens.

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About 17-ID-2 FMX Beamline

The Frontier Microfocusing Macromolecular Crystallography (FMX) delivers a flux of 3.5x1012 ph/s at 1 Å wavelength into a 1x1.5 to 10x10 µm2 (VxH) variable beam focus, covering an energy range from 5 - 30 keV.

Its flux density surpasses current MX beamlines by up to two orders of magnitude.

The FMX optical system features a highly stable horizontal bounce Si 111 double crystal monochromator, as well as 16-segment bimorph bending mirrors in a single stage vertical and a dual stage horizontal focusing scheme.

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About 16-ID LiX Beamline

The Life Science X-ray Scattering (LiX) beamline currently supports experiments in two scientific areas: biomolecular solution scattering and microbeam diffraction from biological tissues.

For solution scattering, several modes of measurements are available.

During routine operations, we can switch instantaneously between high throughput static measurements and in-line size exclusion chromatography (SEC), both of which are based on flow cells.

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About 17-BM Partner User Beamline

The X-ray Footprinting of Biological Materials (XFP) beamline, operated by Case Western Reserve University in partnership with NSLS-II, provides access to synchrotron X-ray footprinting. This solution-state method uses X-ray radiolysis of water to produce reactive hydroxyl radicals that can then react with solvent-accessible regions of proteins and nucleic acids.

This provides a probe of the relative solvent accessibility of different regions of a macromolecule in response to structural changes during binding or kinetic transformations. Two experimental modes are supported providing access to a broad span of total X-ray doses and sample types.

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About 19-ID NYX Partner User Beamline

The Biological Microdiffraction Facility beamline, also called NYX beamline, has the scientific objective of providing a state-of-the-art source for a wide spectrum of crystallographic experiments and a cutting-edge source for optimized anomalous diffraction experiments.

While moving to incorporate microbeams, we also want to preserve the versatility of optimized anomalous scattering experiments across a broad spectrum of atomic resonances from different elements. In order to carry out these experiments and research, we capitalize on the intrinsic brightness of NSLS-II to optimize anomalous signals by improving energy resolution

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