General Lab Information

Erik Farquhar

XFP Beamline Scientist, NSLS-II

Erik Farquhar

Brookhaven National Laboratory

NSLS-II
Bldg. 745, Room 106
P.O. Box 5000
Upton, NY 11973-5000

(631) 344-8174
efarquhar@bnl.gov

Erik is resident beamline scientist for the X-ray Footprinting of Biological Materials (XFP, 17-BM) beamline, a role that he has held since 2018. XFP is operated by the Case Western Reserve University School of Medicine as a Partner Beamline at the National Synchrotron Light Source II, and is dedicated to synchrotron X-ray footprinting, an emerging method to probe the structure and dynamics of biomolecules in the native solution state. 

Previously, Erik led user support for a biological X-ray absorption spectroscopy PRT program at the NSLS from 2010-2014, folllowed by major contributions to the construction and commissioning of the XFP beamline in 2015-2017.

Education | Publications

Education

  • Ph.D. 2010: Inorganic Chemistry, University of Minnesota, Minneapolis, MN USA
  • B.S. 2002: Chemistry, University at Buffalo (SUNY), Buffalo, NY USA

Selected Publications

  • Jain R, Farquhar ER, Dhillon NS, et al (2024) Multiplex Trifluoromethyl and Hydroxyl Radical Chemistry Enables High-Resolution Protein Footprinting. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.4c04610
  • Farquhar ER, Vijayalakshmi K, Jain R, et al (2023) Intact mass spectrometry screening to optimize hydroxyl radical dose for protein footprinting. Biochemical and Biophysical Research Communications 671:343–349. https://doi.org/10.1016/j.bbrc.2023.06.020
  • Jain R, Dhillon NS, Farquhar ER, et al (2022) Multiplex Chemical Labeling of Amino Acids for Protein Footprinting Structure Assessment. Analytical Chemistry 94:9819–9825. https://doi.org/10.1021/acs.analchem.2c01640
  • Jain R, Abel D, Rakitin M, et al (2021) New high-throughput endstation to accelerate the experimental optimization pipeline for synchrotron X-ray footprinting. Journal of Synchrotron Radiation 28:1321–1332. https://doi.org/10.1107/s1600577521005026
  • Asuru A, Farquhar ER, Sullivan M, et al (2019) The XFP (17-BM) beamline for X-ray footprinting at NSLS-II. Journal of Synchrotron Radiation 26:1388–1399. https://doi.org/10.1107/s1600577519003576
  • Schwalenstocker K, Paudel J, Kohn AW, et al (2016) Cobalt Kβ valence-to-core X-ray emission spectroscopy: a study of low-spin octahedral cobalt(iii) complexes. Dalton Transactions 45:14191–14202. https://doi.org/10.1039/c6dt02413k
  • Kiser PD, Farquhar ER, Shi W, et al (2012) Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis. Proceedings of the National Academy of Sciences 109:. https://doi.org/10.1073/pnas.1212025109
  • Wang D, Farquhar ER, Stubna A, et al (2009) A diiron(iv) complex that cleaves strong C–H and O–H bonds. Nature Chemistry 1:145–150. https://doi.org/10.1038/nchem.162
  • Emerson JP, Kovaleva EG, Farquhar ER, et al (2008) Swapping metals in Fe- and Mn-dependent dioxygenases: Evidence for oxygen activation without a change in metal redox state. Proceedings of the National Academy of Sciences 105:7347–7352. https://doi.org/10.1073/pnas.0711179105
Erik Farquhar

Brookhaven National Laboratory

NSLS-II
Bldg. 745, Room 106
P.O. Box 5000
Upton, NY 11973-5000

(631) 344-8174
efarquhar@bnl.gov

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