Technologies Available for License

2002-021: Media for suppression of Auto-induction in Expression Strains

Invention: 2002-021

Patent Status: U.S. Patent Number 8,241,887 was issued on August 14, 2012

For technical and licensing related questions, email tcp@bnl.gov.

Summary

enlarge

F. William Studier

Media for maintenance growth of T7 gene expression strains carrying expression constructs with suppression of unintended induction are provided in this patent. The unintended induction of expression strains is the cause of loss of the expression plasmid from the cells, such that all surviving cells lack the gene expression construct. The media and methods of this invention avoids unintended expression and results in preservation of the the expression strain, even for genes encoding toxic proteins.

Description

In T7 expression host strains, any basal level expression of the T7 RNA polymerase (unintended induction) may lead to loss of the expression construct from the cells. To avoid this, special defined media and methods for their use have been developed and are covered in this patent. The various media are available commercially or may be made from the details in the patent specification.

Journal Publication & Intellectual Property

• Protein production by auto-induction in high-density shaking cultures (.pdf)
• US 8,241,887 B2 (.pdf)

Press Releases

• Brookhaven Lab Biophysicist F. William Studier Wins R&D 100 Awared for A New Method that Simplifies Protein Production (.pdf)