General Lab Information

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User Laboratories

NSLS-II has a number of wet and dry labs available for users of the facility. Minimum Personnel Protective Equipment (PPE) requirements include long pants and closed-toed shoes. In order to access any of the labs General Users must request the lab(s) on their beam time Safety Approval Form (SAF). Staff members and local users must have their lab activity included either in a lab Experimental Safety Review (ESR) form or on a beam time SAF. All lab access, including key-card encoding, lab orientation, and equipment training will be arranged through the lab’s Cognizant Space Manager (CSM).

Lab Locations

There are seven labs located in Building 741 (LOB 1) and nine labs located in Building 743 (LOB 3). We recommend that users requiring a lab contact the lab’s CSM as early as possible to discuss their desired lab activities.

LOB locations

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LOB 1 (1L)

ROOM LAB NAME LAB PHONE CONTACT (CSM) CONTACT PHONE
1L-L04 Ops-CSX-XPD-ESM 631-344-3154 Ryan Hollmers 631-344-7571
1L-L09 Battery & Condensed Matter Lab 631-344-3080 Christine Ali 631-344-3197
1L-L10 Infrared Lab 631-344-8738 Randy Smith 631-344-8033

1L-L04 Ops-CSX-XPD-ESM

Lab 1L-L04 is a dry lab intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components. Solvents may be used in very small quantities. Chemicals and hazardous materials or samples (e.g. nanoparticles) are not permitted.

1L-L06 Dry Lab for Beamline Assembly

Lab 1L-L06 is a dry lab that is used for long term (months) requests for beamline and/or instrument assembly tasks. Requests to use this lab must be made well in advance of the time it will be needed.

1L-L09 Battery & Condensed Matter Lab

Managed by Diffraction & In-situ Scattering program (XPD, PDF, ISR, HEX)

Lab 1L-L09 is a wet lab designed for operations and General User support primarily for the XPD, PDF, ESM, CSX-1, CSX-2 beamlines. This is shared space that includes miscellaneous manipulation and mounting tools for powder and/or single crystal samples: a Mettler AX205 Analytical Semi Micro Balance (81-220 g range, 0.01 mg accuracy), a Wild Heerbrugg M5A Stereo microscope, a Fisher micro centrifuge (Model 235A), a Stabil-Thermo Gravity Oven (model OV-18A, Max Temperature 288°C), a forced-air oven by Lab Safety (Model 32EZ27, max temperature 250°C, +/- 1°C stability), a Corning hot plate stirrer (model PC351), an UltraSonik 57X ultrasonic bath cleaner that has heating capabilities, a Tektronix TAS 455 two-channel Oscilloscope, an Agilent 33220A 20 Mhz waveform generator, a pellet maker, an Orbital Shaker with Sieve for filtering powders, and a variety a lab tools e.g. tweezers, spatulas, cutters, agate mortar and pestle, glassware, double-sided tape, Kapton film/tape, gloves, kimwipes, pipets, copper and aluminum foils, and Kapton/glass capillary containers. The lab has a bench top for wet chemistry with exposure control provided by a Fume Hood with HEPA filter (Air Sentry) and a MilliQ ultra pure water system.

1L-L10 Infrared Lab

Lab 1L-L10 is a wet lab designed for (1) Mid- and Far-IR ellipsometery of condensed matter materials (MET program), (2) High Pressure IR, Raman, & vis fluorescent Microspectroscopy (FIS program), and (3) FTIR Microspectroscopy/Imaging. For mid & Far-IR ellipsometery, the lab is equipped with a Bruker 66 vacuum FTIR spectrometer, a vacuum mid/far IR ellipsometer custom built by NJIT, and several mid- and far- IR detectors. For high pressure work, the Carnegie Institution of Washington has developed an experiment station to accommodate Diamond Anvil Cells (DAC) for FTIR spectroscopy uses a Bruker vertex80/Hyperion microscope, and a custom built setup for ruby-pressure-calibration and other Raman/fluorescence measurements. For FTIR Microspectroscopy/Imaging, the lab is equipment with a PerkinElmer Spectrum Spotlight 300, a Vertex 80 + Hyperion 3000 w/ FPA, and a Thermo Magna/Continuum FTIR microscope. Use of the IR lab requires specialized training prior to use.

LOB 3 (3L)

ROOM LAB NAME LAB PHONE CONTACT (CSM) CONTACT PHONE
3L-L01 Imaging and Microscopy Dry Lab 631-344-6094 Randy Smith 631-344-8033
3L-L04 Ops-IXS, SIX, ISR, SST 631-344-7680 Ryan Hollmers 631-344-7571
3L-L05A Cryo Imaging Prep Randy Smith 631-344-8033
3L-L06 Spectroscopy Dry Prep 631-344-6090 Syed Khalid 631-344-7496
3L-L07 Environmental Sciences Lab 631-344-4535 Randy Smith 631-344-8033
3L-L08 Mesoscale Imaging Wet Lab 631-344-3719 Randy Smith 631-344-8033
3L-L09 XAS Chemistry Lab 631-344-3176 Syed Khalid 631-344-7496

3L-L01 Imaging and Microscopy Dry Lab

Lab 3L-L01 is dry lab for basic sample preparation and mounting for users of the HXN, SRX, XFM, and FXI beamlines. The lab has sample manipulation and mounting tools such as tweezers, cutters, double-sided tape, Kapton film, and ultralene film. It is also equipped with a Leica SD optical microscope w/ digital camera for sample inspection, a HEPA laminar flow hood for clean sample prep, a refrigerator for sample storage, and a vacuum oven for sample drying. Lab 3-LL01 has designated user sample prep space for each of the 4 beamlines in the center of the lab and shared workspace around the perimeter.

3L-L02 Dry Lab for ISS, TES, QAS, & BMM

Lab 3L-L02 is a dry lab is intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components. Solvents may be used in very small quantities. Hazardous chemicals or materials are not permitted. This lab has a soldering station, a LASER and 3D printer is also available. A SentroTech, high temperature (1200 deg. C), vacuum furnace, with possible Argon venting or atmosphere is also available. Personal Protection Equipment (PPE) is provided for safe handling of any work done here.

3L-L04 Ops-IXS, SIX, ISR, SST

Lab 3-LL04 is a dry lab for basic sample preparation and mounting for beamline staff and users of the ISR, IXS, SIX, and SST beamlines. The lab has a five foot Purifier Horizontal Clean Bench hood, Leica M205 C Stereomicroscope, cooling water, and soldering station. If needed, 60 L dewars of liquid helium or nitrogen may be used in this lab. Each beamline has designated space: 4’ workbench with overhead task lighting, storage cabinets and drawers. The beamlines share countertop workspace around the perimeter, assembly table (60 in. X 32 in.), and beryllium storage cabinet.

3L-L06 Spectroscopy Dry Prep

3LL06 is a dry XAS sample preparation laboratory, mostly used by ISS, QAS and BMM beamlines. The lab has three stations for sample preparation, each includes a balance, a press, 13 mm die, mortar and pestle. 5 mm, 1 and 1.5-inch diameter dies can also be arranged. Wig-L-Bug sample grinder, an oven, a mylar bag heat sealer and a low magnification optical microscope are also available. The lab has few sample binders (BC, BN, zeolite, polyethylene glycol, graphite powder, carbon black) and general organic cleaning chemicals. A soldering station is also available. If trained, users can dispose hazardous chemical waste here. Personal Protection Equipment (PPE) is provided for safe handling of any work done here.

3L-L07 Environmental Sciences Lab

Managed by Imaging and Microscopy program (HXN, SRX, FXI, XFM)

The principle purpose of Lab 3L-L07 is wet chemistry. It is configured for environmental work with trace elements, toxic metals, and hazardous substances. As such, additional PPE (personal protection equipment) requirements may be required. Equipment available to users includes: 18.2 MegaOhm water source, glove box with dry nitrogen (N2) atmosphere*, orbital shaker, sample oven, analytical balance, high-capacity balance, digital pipettes, tabletop centrifuge, USDA standard sieves, pH electrodes and meters, sonicators, desiccators, freeze-drier*, vortexer, Leica stereomicroscope with digital camera, computer with internet access, fluorescent light bank for plant cultivation, cell culture hood, refrigerator*, circulator bath, hot/stir plates, lab glassware, lab jacks/stands and consumables (e.g., basic chemicals & reagents, LN2, gloves, goggles, Kimwipes, etc.). Reagent and LN2 use requires training.

3L-L08 Mesoscale Imaging Wet Lab

Managed by Imaging and Microscopy program (HXN, SRX, FXI, XFM)

The principle capabilities for Lab 3L-L08 include (1) sample preparation and (3) visible microscopy characterization for imaging and (3) a mammalian cell culture facility. For sample preparation, the lab is equipment with two Leica microtomes* (ambient and low temperature) and a Leica plunge freezer*. For visible microscopy characterization the lab has two upright stereo microscopes: a Nikon Eclipse microscope with bright-field transmission, epifluorescence, and dark field viewing modes, digital camera, and motorized stage; and an Olympus BX51 with bright-field & epifluorescence viewing modes and a digital camera. For mammalian cell culture*, the lab is equipped with a cell culture hood, incubator, autoclave, -80 freezer, LN2 storage dewar, and cytospin. *Requires training

3L-L09 XAS Chemistry Lab

3LL09 is a wet XAS-chemistry lab, frequently used by ISS, QAS and BMM, and sometimes used by ISR, SST-1, SST-2, TES, CHX, FXI, XPD and PDF beamlines. Capable of doing fuel cell and battery work. It has an Argon atmosphere glove box, available by special arrangement, and limited HF handling capabilities. It is equipped with a balance, hot press, an oven, a vacuum oven, table top centrifuge, ultrasonic cleaner, hot plate with stirrer, pH meter, low magnification optical microscope, de-ionized water, about 180 chemicals, and a desk top computer. If trained, users can dispose hazardous chemical waste in this lab. It has a HEPA filtered hood, cabinets to store acids and flammable chemicals. Personal Protection Equipment (PPE) is provided for safe handling of any work done here.

LOB 4 (4L)

ROOM LAB NAME LAB PHONE CONTACT (CSM) CONTACT PHONE
4L-L04 Soft Matter Dry Lab 631-344-6347 Lutz Wiegart 631-344-8064
4L-L08 Soft Matter Chem Lab 631-344-6341/6342 Christine Ali 631-344-3197

4L-L04 Soft Matter Dry Lab for CHX, IXS, SMI, SST-1 & CMS

This laboratory is designed for the assembly, setup, and testing of sample environmental chambers and ancillary equipment, inspection and non-x-ray characterization of samples, and commissioning of beamline equipment. Electronic prototyping and assembly may be performed, involving small low voltage electronic circuits, as well as, ultra-high vacuum equipment testing. Some equipment listed for use in the lab space is as follows: Carbon Dioxide snow jet cleaner, Nikon Microscope, temperature-controlled oven, and a movable snorkel exhaust. Class 2 lasers may be brought in as needed. Solvents may be used in small quantities.

4L-L08 Soft Matter Chemistry Lab for CHX, IXS, SMI, SST-1, & CMS

Laboratory 4LL08 is a multi-purpose chemistry lab available for use by trained staff members, their collaborators, and NSLS-II users involved in soft matter research. The lab is set up for chemistry related sample preparation and characterization using both aqueous and organic solutions. Sample cleaning via organic solvents, Hydrochloric and Nitric acid, and piranha etch solutions may be performed following defined work instructions. Some equipment available in the lab are two HEPA filtered fume hoods, a compact muffle furnace (1100◦C), a G3P-8 spin coating system, a Dataphysics contact angle system, a nitrogen-gas glove box, and a vacuum oven. The laboratory is equipped with balances, vortex mixer, a Millipore Milli-Q water purification system, pH meters, stirrer/hot plates, centrifuge, ultrasonic bath, stereo microscope, refrigerator/freezer, ice maker, pipettes, glassware and general laboratory consumables including common solvents. Solvents and acids are provided to avoid the complications associated with DOT shipping regulations.

LOB 5 (5L)

ROOM LAB NAME LAB PHONE CONTACT (CSM) CONTACT PHONE
5L-L06 Structural Biology Lab 631-344-6351/6352 Christine Ali 631-344-3197
5L-L07 Cryo-EM Sample Prep Lab 631-344-6359 Guobin Hu 631-344-7915
5L-L08 Sample Prep Lab FXI, NYX, XFP 631-344-6358 Mike Sullivan 631-344-3800
5L-L09 Life Sciences Automation Dev. Grace Shea-McCarthy 631-344-2025

5L-L06 Structural Biology Lab for LiX, FMX, AMX, NYX & XFP

Lab 5L-L06 is a Chemistry lab for protein crystallization and biological sample preparation. The laboratory is equipped with balances, vortex, Millipore Milli-Q water purification systems, pH meters, stirrer/hot plate, rocker, UV/VIS spectrophotometer, microcentrifuge, air-driven ultracentrifuge, sonic dismembrator, ultrasonic cleaner, stereo microscope, refrigerator/freezer, fume hood, pipettes, glassware and general laboratory consumables. Some common chemicals and acids/bases such as sodium chloride and hydrochloric acid are stored. Vapor diffusion (15 well) and microbatch (96 well) plates are also available. More specialized equipment, requiring additional equipment and technique training, include a high-speed centrifuge, low pressure protein purification system, isocratic HPLC system, speed vac, and protein electrophoresis system are available. For very small protein volumes (<5 µL per drop) there is a Labcyte ECHO system (acoustic droplet ejection system). The Labcyte ECHO system can also be used to set up plates automatically and go to larger volumes (>5 µL). There are incubators to control the environment in which crystals grow and microscopes with cameras to check plates and to capture images. Additional capabilities include nanodrop and a cold room for temperature sensitive work.

5L-L07 5LL07 Cryo-EM Sample Prep Lab

Lab 5L-L07 is a Chemistry lab for the Cryo-Electron Microscopy (EM) sample preparation. The laboratory is equipped with a carbon coater to deposit carbon film on an EM grid, a glow discharger to render carbon film to be hydrophilic, a Vitrobot to freeze cryo-samples, and a cryo-holder pumping station to warm up and dry the cryo-holder. There is a refrigerator for temporary sample storage, pipettes to prepare and apply samples to EM grids, and a cryo-tank to store cryo-samples.

5L-L08 Sample Prep Lab for FXI, NYX & XFP

Lab 5L-L08 is a dry lab for beamline staff and users of XFP, FXI, and NYX. It is intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components. Solvents may be used in small quantities, and a soldering station is also available. A small oven and a battery cycler are available for use.