Gene Transfer and Genetic Engineering

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Using a genetically engineered DNA (a plasmid) as a vector to carry the genes of interest; students will transfer the code (gene) for jellyfish GFP (green fluorescent protein) and the code for an antibiotic resistant gene, into E. coli. If E.coli is successfully transformed, the cells should express the antibiotic resistant gene and grow in a media that contains antibiotic and express the green fluorescent gene and glow green in the presence of UV light.

Vocabulary: ampicillin, antibiotic, green fluorescent protein (GFP), plasmid, transformation

Session Information: This activity is available in-person only.

In-Person Session Information

1-hour session, times are flexible
One class per session, maximum 30 students per class
Can book multiple activities in one day
$400 (BOCES-aidable) per session

NYS Learning Standards

New York State Science Learning Standards

Disciplinary Core Ideas	Crosscutting Concepts	Science and Engineering Practices
LS1.A: Structure and Function LS3.A: Inheritance of Traits LS3.B: Variation of Traits	Cause and Effect Structure and Function	N/A

Disciplinary Core Ideas

Crosscutting Concepts

Science and Engineering Practices

LS1.A: Structure and Function

LS3.A: Inheritance of Traits

LS3.B: Variation of Traits

Cause and Effect

Structure and Function

N/A

Next Generation English Language Arts Learning Standards

Speaking and Listening	Language
Comprehension and Collaboration Presentation of Knowledge and Ideas	Vocabulary Acquisition and Use

Intermediate Science Core

Process Skills	Major Understandings
Standard 4, 1 Follow safety procedures in the classroom and laboratory. Standard 1, S2.1a Demonstrate appropriate safety techniques. Standard 1, S2.1b Conduct an experiment design by others. Standard 1, S2.1d Use appropriate and conventional techniques to solve problems about the natural world, including: measuring, observing, and describing.	2.1a Hereditary information in contained in genes. Genes are composed of DNA that makes up the chromosomes of cells. 2.1b Each gene carries a single unit of information. A single inherited trait of an individual can be determined by one pair or by many pairs of genes. A human cell contains thousands of different genes. 2.2a In all organisms, genetic traits are passed down from generation to generation. 3.1c Human activities such as selective breeding and advances in genetic engineering may affect the variations of species.

Process Skills

Major Understandings

Standard 4, 1 Follow safety procedures in the classroom and laboratory.

Standard 1, S2.1a Demonstrate appropriate safety techniques.

Standard 1, S2.1b Conduct an experiment design by others.

Standard 1, S2.1d Use appropriate and conventional techniques to solve problems about the natural world, including: measuring, observing, and describing.

2.1a Hereditary information in contained in genes. Genes are composed of DNA that makes up the chromosomes of cells.

2.1b Each gene carries a single unit of information. A single inherited trait of an individual can be determined by one pair or by many pairs of genes. A human cell contains thousands of different genes.

2.2a In all organisms, genetic traits are passed down from generation to generation.

3.1c Human activities such as selective breeding and advances in genetic engineering may affect the variations of species.

Program Topics

biology exploration labs

Program Audience

6th Grade 7th Grade 8th Grade

Gene Transfer and Genetic Engineering

NYS Learning Standards

Contact

Ashley Blydenburgh

About Us

Program Topics

Program Audience

Brookhaven National Laboratory

Brookhaven Science Associates