Gene Transfer and Genetic Engineering

To request field trip dates, visit https://oepreg.bnl.gov/.
Using a genetically engineered DNA (a plasmid) as a vector to carry the genes of interest; students will transfer the code (gene) for jellyfish GFP (green fluorescent protein) and the code for an antibiotic resistant gene, into E. coli. If E.coli is successfully transformed, the cells should express the antibiotic resistant gene and grow in a media that contains antibiotic and express the green fluorescent gene and glow green in the presence of UV light.
Vocabulary: ampicillin, antibiotic, green fluorescent protein (GFP), plasmid, transformation
Session Information: This activity is available in-person only.
In-Person Session Information |
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To request field trip dates, visit https://oepreg.bnl.gov/.
NYS Learning Standards
New York State Science Learning Standards
Disciplinary Core Ideas | Crosscutting Concepts | Science and Engineering Practices |
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LS1.A: Structure and Function LS3.A: Inheritance of Traits LS3.B: Variation of Traits |
Cause and Effect Structure and Function |
N/A |
Next Generation English Language Arts Learning Standards
Speaking and Listening | Language |
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Comprehension and Collaboration |
Vocabulary Acquisition and Use |
Intermediate Science Core
Process Skills |
Major Understandings |
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Standard 4, 1 Follow safety procedures in the classroom and laboratory. Standard 1, S2.1a Demonstrate appropriate safety techniques. Standard 1, S2.1b Conduct an experiment design by others. Standard 1, S2.1d Use appropriate and conventional techniques to solve problems about the natural world, including: measuring, observing, and describing. |
2.1a Hereditary information in contained in genes. Genes are composed of DNA that makes up the chromosomes of cells. 2.1b Each gene carries a single unit of information. A single inherited trait of an individual can be determined by one pair or by many pairs of genes. A human cell contains thousands of different genes. 2.2a In all organisms, genetic traits are passed down from generation to generation. 3.1c Human activities such as selective breeding and advances in genetic engineering may affect the variations of species. |