Gene Transfer and Genetic Engineering

Using a genetically engineered DNA (a plasmid) as a vector to carry the genes of interest,  students will transfer the code (gene) for jellyfish green fluorescent protein (GFP) and the code for an antibiotic-resistant gene, into E. coli. If the transformation is successful, students will witness living proof of their work:  E. coli that not only grow in the presence of antibiotics but also glow green under UV light.

Vocabulary: ampicillin, antibiotic, green fluorescent protein (GFP), plasmid, transformation

To request field trip dates, visit https://oepreg.bnl.gov/.

To explore programs listed in the ESBOCES Exploratory Enrichment catalog and to submit a BOCES contract request, please visit BOCES EE – Brookhaven Lab   

Click here to download a teacher guide for this program.

NYS Learning Standards

New York State Science Learning Standards

Next Generation English Language Arts Learning Standards