General Lab Information


Gene Transfer and Genetic Engineering

program image

Using a genetically engineered DNA (a plasmid) as a vector to carry the genes of interest; students will transfer the code (gene) for jellyfish GFP (green fluorescent protein) and the code for an antibiotic resistant gene, into E. coli.  If E.coli is successfully transformed, the cells should express the antibiotic resistant gene and grow in a media that contains antibiotic and express the green fluorescent gene and glow green in the presence of UV light.  

Vocabulary: ampicillin, antibiotic, green fluorescent protein (GFP), plasmid, transformation

Session Information: This activity is available in-person only. 

In-Person Session Information
  • 1-hour session, times are flexible
  • One class per session, maximum 30 students per class
  • Can book multiple activities in one day
  • $400 (BOCES-aidable) per session

NYS Learning Standards

New York State Science Learning Standards

Disciplinary Core Ideas Crosscutting Concepts Science and Engineering Practices

LS1.A: Structure and Function

LS3.A: Inheritance of Traits

LS3.B: Variation of Traits

Cause and Effect

Structure and Function


Next Generation English Language Arts Learning Standards

Speaking and Listening Language

Comprehension and Collaboration
Presentation of Knowledge and Ideas

Vocabulary Acquisition and Use

Intermediate Science Core

Process Skills

Major Understandings

Standard 4, 1 Follow safety procedures in the classroom and laboratory.

Standard 1, S2.1a Demonstrate appropriate safety techniques.

Standard 1, S2.1b Conduct an experiment design by others.

Standard 1, S2.1d Use appropriate and conventional techniques to solve problems about the natural world, including: measuring, observing, and describing.

2.1a Hereditary information in contained in genes. Genes are composed of DNA that makes up the chromosomes of cells.

2.1b Each gene carries a single unit of information. A single inherited trait of an individual can be determined by one pair or by many pairs of genes. A human cell contains thousands of different genes.

2.2a In all organisms, genetic traits are passed down from generation to generation.

3.1c Human activities such as selective breeding and advances in genetic engineering may affect the variations of species.

Program Topics

biologyexploration labs

Program Audience

6th Grade7th Grade8th Grade