General Lab Information

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Gene Transfer and Genetic Engineering (AP Lab)

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Students will transfer green fluorescent protein and antibiotic resistant genes into E. coli by using genetically engineered plasmid DNA as a vector to carry the genes. The transformed E.coli is expected to grow in a media that contains antibiotic and glow green in the presence of ultraviolet light. 

Session Information: 

  • Flexible instruction whether students are in the classroom or at home. 
  • Interactive, instructor-led session facilitated with students using materials from school or home.  Can also be demonstration-based if students do not have access to materials.  
  • Teachers will receive a detailed Teacher Notes guide upon booking.  
  • Secure link to ZoomGov videoconferencing platform will be provided. 
  • $480 (BOCES-aidable) 
  • Time: 2 hours
  • Contact us to schedule your field trip 

New York State Science Standards

Core Document Living Environment Science Core
Inquiry  
Performance Indicators PI 1.1 Scientific Inquiry:
“Elaborate on basic scientific and personal explanations of natural phenomena, and develop extended visual models and mathematical formulations to represent one’s thinking.”
PI 3.1 Scientific Inquiry:
“Use various methods of representing and organizing observations (e.g. diagrams, tables, charts, graphs, equations, matrices).”
Major Understandings MU 1.1b, Scientific Inquiry:
“Learning about the historical development of scientific concepts or about individuals who have contributed to scientific knowledge provides a better understanding of scientific inquiry and the relationship between science and society.”
MU 3.1a, Scientific Inquiry:
“Interpretation of data leads to development of additional hypotheses, the formulation of generalizations, or explanations of natural phenomena.”
Content  
Performance Indicators PI 2.1, Living Environment:
“Explain how the structure and replication of genetic material result in offspring that resemble their parents.”
PI 2.2, Living Environment:
“Explain how the technology of genetic engineering allows humans to alter genetic makeup of organisms.”
Major Understandings MU 2.1 b, f, g, h Living Environment:
  • “Every organism requires a set of coded instructions for specifying its traits.”
  • “In all organisms, the coded instructions for specifying the characteristics of the organism are carried in DNA, a large molecule formed from subunits arranged in a sequence with bases of four kinds (represented by A, G, C, and T). The chemical and structural properties of DNA are the basis for how the genetic information that underlies heredity is both encoded in genes (as a string of molecular “bases”) and replicated by means of a template.”
  • “Cells store and use coded information. The genetic information stored in DNA is used to direct the synthesis of the thousands of proteins that each cell requires.”
  • “Genes are segments of DNA molecules.”
MU 2.2 b Living Environment:
“In recent years new varieties of farm plants and animals have been engineered by manipulating their genetic instructions to produce new characteristics.”