Brookhaven National Laboratory
Purpose & Scope of Workshop:
To train researchers in the use of circular dichroism (CD) spectroscopy and closely related approaches for structural and functional characterization of proteins and nucleic acids in solution.
Lectures will include principles of CD and its application to the analysis of the structure of globular proteins, membrane-bound proteins and nucleic acids. Related experimental methods such as, linear dichroism, magnetic circular dichroism and time resolved (stopped flow) CD will be discussed.
Laboratory sessions will cover sample preparation, calibration and data collection using both conventional table-top and synchrotron-source (SRCD) spectrometers, web resources for analysis of protein structures and time resolved (stopped-flow) CD. Participants may bring their own samples that (subject to NSLS procedures) may be examined if time permits. Participants are encouraged to bring laptop computers for use in data analysis.
Graduate, postdoctoral and research scientists interested in using ultraviolet CD spectroscopy to study the structure and conformation of proteins and other biopolymers. Attendance will be limited to approximately 12 participants.
Participants are responsible for travel costs to and from BNL. A registration fee of 400 USD applies to participants requiring dormitory style on-site housing (up to 5 nights) and 235 USD for those who do not require housing. Lunch and two coffee breaks each day are included with both these registration packages. A special registration fee of 150 USD that does not include food services is available only to participants who are BNL employees.
The Biology and National Synchrotron Light Source Departments at Brookhaven National Laboratory, are funded respectively by the Office of Biological and Environmental Research and the Office of Basic Energy Sciences, both units of the Office of Science, U.S. Department of Energy.
Non-US citizens or permanent residents: 9 May
US Citizens: 1 June