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Science

On this page you will find examples of research projects carried out by X9 users.
The examples here may be out-dated. See the publication list for the most recent results.

1. Protein solution scattering

  • Example 1: Determination of dimer dissociation constant kD for SARS virus protease
  • The dimer dissociation constant of a dimeric protein is determined from solution scattering data from solutions at a series of protein concentrations. The scattering data depend on the dimer/monomer population in the solution, which in turn is a function of the protein concentration.

    publication:
    Graziano et.al. SARS CoV Main Proteinase: The Monomer−Dimer Equilibrium Dissociation Constant
    Biochemistry, 2006, 45(49), pp 14632–14641

  • Example 2: Conformation change of Gelsolin as a function of [Ca2+]
  • To follow the global structural changes that accompany calcium activation of gelsolin, solution scattering data were collected for full-length human plasma gelsolin at nanomolar to millimolar concentrations of free Ca2+. Analysis of these data showed that, upon increasing free Ca2+ levels, the radius of gyration (Rg) increased nearly 12 Å, from 31.1 ± 0.3 to 43 ± 2 Å, and the maximum linear dimension (Dmax) of the gelsolin molecule increased 55 Å, from 100 to 155Å. Structural reconstruction of gelsolin from these data provided a striking visual tracking of the gradual Ca2+-induced opening of the gelsolin molecule and highlighted the critical role played by the flexible linkers between homologous domains.

    publication:
    Ashish et.al. Global Structure Changes Associated with Ca2+ Activation of Full-length Human Plasma Gelsolin
    The Journal of Biological Chemistry, 282, pp 25884-25892.

2. Self-assembly

3. Lipid membrane structures

4. Structure of organic semiconductors