National Synchrotron Light Source II
NSLS-II is a state-of-the-art 3 GeV electron storage ring. The facility offers scientific and industrial researchers an array of beamlines with x-ray, ultraviolet, and infrared light to enable discoveries in clean and affordable energy, high-temperature superconductivity, molecular electronics, and more. Overview »
The next deadline for NSLS-II beam time proposals and beam time requests is September 30, 2018. | Submission Details
Become a Facility User
Beamlines at the National Synchrotron Light Source II are open to academic and industrial users for scientific research. All research proposals are subjected to peer review and ranked against competing proposals based on scientific merit.
Beamline Projects
When fully built out, National Synchrotron Light Source II will accommodate approximately 60 to 70 beamlines. Thirty beamlines are currently in various stages of development and will provide significant research capacity in early operations.
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Seminars
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OCT
11
Thursday
NSLS-II Colloquium Series
"Biophysical Studies of an RNA Virus particle and its Maturation: Insights into an Elegantly Programmed Nano-machine"
Presented by John E. (Jack) Johnson, Department of Integrative Structural and Computational Biology, The Scripps Research Institute
4 pm, Large Seminar Room, Bldg. 510
Thursday, October 11, 2018, 4:00 pm
Hosted by: John Hill
Nudaurelia Capensis ? Virus (N?V) is a eukaryotic, quasi-equivalent, RNA virus, with a T=4 surface lattice, where maturation is dramatic (a change in particle size of 100Å) and is novel in that it can be investigated in vitro. Here we use X-ray crystallography, biochemistry, Small Angle X-ray Scattering, and electron cryo-microscopy and image reconstruction (CryoEM), to characterize maturation intermediates, an associated auto-catalytic cleavage, the kinetics of morphological change and to demonstrate that regions of N?V subunit folding are maturation-dependent and occur at rates determined by their quasi-equivalent position in the capsid. Matsui, T., Lander, G. C., Khayat, R., and Johnson, J. E. 2010. Subunits fold at position-dependent rates during maturation of a eukaryotic RNA virus. Proc Natl Acad Sci U S A 107:14111-5. Veesler, D., and Johnson, J.E. 2012. Virus Maturation. Annual review of biophysics 41:473-496. Doerschuk, P. C., Gong, Y., Xu, N., Domitrovic, T., and Johnson, J. E. 2016. Virus particle dynamics derived from CryoEM studies. Curr Opin Virol 18:57-63.
