Magnet Test Database Workshop (May 7, 2018)
2nd International Magnet Test Stand Workshop (May 8-9, 2018)
Proceedings of the 1968 Summer Study on Superconducting Devices and Accelerators
JUN
28
Wednesday
Center for Biomolecular Structure Lecture Series
"Structural Photophysics of Fluorescent Proteins Used in Super Resolution Microscopy"
Presented by Dominique Bourgeois, IBS, France
1:30 pm, Videoconference / Virtual Event
Wednesday, June 28, 2023, 1:30 pm
Hosted by: Vivian Stojanoff
Phototransformable fluorescent proteins (PTFPs) are widely used in many advanced fluorescence microscopy methods, notably in the super-resolution field. A plethora of PTFPs have been engineered in the last years, which display a variety of photoactivation, photoconversion, photoswitching, photoblinking and photobleaching properties that can be used at advantage in many super-resolution schemes, but that are also at the origin of significant complications and artifacts, making these fascinating genetically encoded labels still far from ideal. In this talk, I will review how (kinetic) X-ray crystallography, optical spectroscopy, multidimensional NMR and molecular dynamics simulations can be combined with single-molecule investigations to reveal phototransformation mechanisms of PTFPs at the near-atomic level.