Meetings & Workshops
Magnet Test Database Workshop (May 7, 2018)
2nd International Magnet Test Stand Workshop (May 8-9, 2018)
Proceedings of the 1968 Summer Study on Superconducting Devices and Accelerators
Talks
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JUN
28
Wednesday
Center for Biomolecular Structure Lecture Series
"Structural Photophysics of Fluorescent Proteins Used in Super Resolution Microscopy"
Presented by Dominique Bourgeois, IBS, France
1:30 pm, Videoconference / Virtual Event
Wednesday, June 28, 2023, 1:30 pm
Hosted by: Vivian Stojanoff
Phototransformable fluorescent proteins (PTFPs) are widely used in many advanced fluorescence microscopy methods, notably in the super-resolution field. A plethora of PTFPs have been engineered in the last years, which display a variety of photoactivation, photoconversion, photoswitching, photoblinking and photobleaching properties that can be used at advantage in many super-resolution schemes, but that are also at the origin of significant complications and artifacts, making these fascinating genetically encoded labels still far from ideal. In this talk, I will review how (kinetic) X-ray crystallography, optical spectroscopy, multidimensional NMR and molecular dynamics simulations can be combined with single-molecule investigations to reveal phototransformation mechanisms of PTFPs at the near-atomic level.
