NSLS-II has a number of wet and dry labs available for users of the facility. Minimum Personnel Protective Equipment (PPE) requirements include long pants and closed-toed shoes. In order to access any of the labs General Users must request the lab(s) on their beam time Safety Approval Form (SAF). Staff members and local users must have their lab activity included either in a lab Experimental Safety Review (ESR) form or on a beam time SAF. All lab access, including key-card encoding, lab orientation, and equipment training will be arranged through the lab’s Cognizant Space Manager (CSM).
There are seven labs located in Building 741 (LOB 1) and nine labs located in Building 743 (LOB 3). We recommend that users requiring a lab contact the lab’s CSM as early as possible to discuss their desired lab activities.
|ROOM||LAB NAME||LAB PHONE||CONTACT (CSM)||CONTACT PHONE|
|1L-L04||Dry Lab for XPD, ESM & CSX||631-344-3154||Andi Barbour||631-344-3895|
|1L-L05||Dry Lab for FMX, AMX, LiX||631-344-3249||Grace Shea-McCarthy||631-344-2025|
|1L-L06||Dry Lab for Beamline Assembly||631-344-3284||Andi Barbour||631-344-3895|
|1L-L07||Soft & Bio Materials Wet Lab||631-344-3429||Vito Graziano||631-344-2209|
|1L-L08||Structural Biology Wet Lab||631-344-3442||Edwin Lazo||631-344-7247|
|1L-L09||Battery & Condensed Matter Lab||631-344-3080||John Trunk||631-344-3406|
|1L-L10||Infrared Lab||631-344-8738||Randy Smith||631-344-8033|
Lab 1L-L04 is a dry lab intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components. Solvents may be used in very small quantities. Chemicals and hazardous materials or samples (e.g. nanoparticles) are not permitted.
Lab 1L-L05 is a dry lab that is being used for beamline assembly until June 1, 2016. After that date, this lab is intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components for the ABBIX beamlines. Solvents may be used in very small quantities. Chemicals and hazardous materials or samples (e.g. nanoparticles) are not permitted.
Lab 1L-L06 is a dry lab that is used for long term (months) requests for beamline and/or instrument assembly tasks. Requests to use this lab must be made well in advance of the time it will be needed.
Lab 1L-L07 is a wet lab for conducting scientific research in soft matter and biological sample preparation. Common laboratory equipment includes a balance, pH meter, stirrer/hot plate, rocker, UV/VIS spectrophotometer, vortexer, microcentrifuge, pipettes, air-driven ultracentrifuge, sonic dismembrator, ultrasonic cleaner, stereo microscope, microwave, oven, refrigerator/freezer, HEPA filter fume hood, MilliQ water purification system, glassware and general laboratory consumables. More specialized equipment, requiring additional equipment and technique training, include a high speed centrifuge, low pressure protein purification system, isocratic HPLC system, speed vac, and protein electrophoresis system.
Lab 1L-L08 is a wet lab for protein crystallization. The laboratory is equipped with balances, vortex, Millipore water purifier, pH meters, and pipettes to prepare your protein sample and all necessary buffers. Some common chemicals and acids/bases such as sodium chloride and hydrochloric acid are stored. Vapor diffusion (15 well) and microbatch (96 well) plates are also available. For very small protein volumes (<5 µL per drop) there is a Labcyte ECHO system (acoustic droplet ejection system). The Labcyte ECHO system can also be used to set up plates automatically and go to larger volumes (>5 µL). There are incubators to control the environment in which crystals grow and microscopes with cameras to check plates and to capture images. Additional capabilities include circular dichroism and ultra violet spectrometers.
Lab 1L-L09 is a wet lab designed for (1) beamline operations and General User support primarily for XPD-1, XPD-2, ESM, CSX-1, CSX-2 beamlines and (2) battery research support. This is shared space that includes miscellaneous manipulation and mounting tools for powder and/or single crystal samples: a Mettler AX205 Analytical Semi Micro Balance (81-220 g range, 0.01 mg accuracy), a Wild Heerbrugg M5A Stereo microscope, a Poly Science water bath, a Fisher micro centrifuge (Model 235A), a Stabil-Thermo Gravity Oven (model OV-18A, Max Temperature 288°C), a forced-air oven by Lab Safety (Model 32EZ27, max temperature 250°C, +/- 1°C stability), a Corning hot plate stirrer (model PC351), an UltraSonik 57X ultrasonic bath cleaner, a Tektronix TAS 455 two-channel Oscilloscope, an Agilent 33220A 20 Mhz waveform generator, and a variety a lab tools e.g. tweezers, spatulas, cutters, agate mortar and pestle, glassware, double-sided tape, Kapton film/tape, gloves, kimwipes, pipets, copper and aluminum foils, and Kapton/glass capillary containers. It is also equipped for air and/or moisture sensitive material handling and battery cell assembly using an Argon filled glove box (VAC, OMN-LAB). The lab has a bench top for wet chemistry with exposure control provided by a Fume Hood with HEPA filter (Air Sentry) and an electrochemical test of battery cells (Biologic battery cyclers, under procurement).
Lab 1L-L10 is a wet lab designed for (1) Mid- and Far-IR ellipsometery of condensed matter materials (MET program), (2) High Pressure IR, Raman, & vis fluorescent Microspectroscopy (FIS program), and (3) FTIR Microspectroscopy/Imaging. For mid & Far-IR ellipsometery, the lab is equipped with a Bruker 66 vacuum FTIR spectrometer, a vacuum mid/far IR ellipsometer custom built by NJIT, and several mid- and far- IR detectors. For high pressure work, the Carnegie Institution of Washington has developed an experiment station to accommodate Diamond Anvil Cells (DAC) for FTIR spectroscopy uses a Bruker vertex80/Hyperion microscope, and a custom built setup for ruby-pressure-calibration and other Raman/fluorescence measurements. For FTIR Microspectroscopy/Imaging, the lab is equipment with a PerkinElmer Spectrum Spotlight 300, a Vertex 80 + Hyperion 3000 w/ FPA, and a Thermo Magna/Continuum FTIR microscope. Use of the IR lab requires specialized training prior to use.
|ROOM||LAB NAME||LAB PHONE||CONTACT (CSM)||CONTACT PHONE|
|3L-L01||Dry Lab for HXN, SRX, XFM, FXI||631-344-6094||Petr Ilinski||631-344-8049|
|3L-L02||Dry Lab for ISS, TES, QAS, BMM||631-344-5883||Syed Khalid||631-344-7496|
|3L-L03||ROS group/ Detectors||361-344-6092||Qing-Yi Dong||631-344-5358|
|3L-L04||Dry Lab for IXS, SIX, ISR, SST||631-344-7680||Steve LaMarra||631-344-5312|
|3L-L05||Dry Lab for SMI, CMS, CHX||631-344-3540||Yugang Zhang||631-344-5674|
|3L-L06||Beamline Assembly Lab||631-344-6090||Syed Khalid||631-344-7496|
|3L-L07||Environmental Sciences Wet Lab||631-344-4535||Vito Graziano||631-344-2209|
|3L-L08||Mesoscale Imaging Wet Lab||631-344-3719||Vito Graziano||631-344-2209|
|3L-L09||Chemistry Wet Lab||631-344-3176||Syed Khalid||631-344-7496|
Lab 3L-L01 is dry lab for basic sample preparation and mounting for users of the HXN, SRX, XFM, and FXI beamlines. The lab has sample manipulation and mounting tools such as tweezers, cutters, double-sided tape, Kapton film, and ultralene film. It is also equipped with a Leica SD optical microscope w/ digital camera for sample inspection, a HEPA laminar flow hood for clean sample prep, a refrigerator for sample storage, and a vacuum oven for sample drying. Lab 3-LL01 has designated user sample prep space for each of the 4 beamlines in the center of the lab and shared workspace around the perimeter.
Lab 3L-L02 is a dry lab is intended for activities such as sample mounting, set up and testing of experimental apparatuses, assembly of small parts, and testing of beamline components. Solvents may be used in very small quantities. Chemicals and hazardous materials or samples (e.g. nanoparticles) are not permitted.
Lab 3-LL04 is a dry lab for basic sample preparation and mounting for beamline staff and users of the ISR, IXS, SIX, and SST beamlines. The lab has a five foot Purifier Horizontal Clean Bench hood, Leica M205 C Stereomicroscope, cooling water, and soldering station. If needed, 60 L dewars of liquid helium or nitrogen may be used in this lab. Each beamline has designated space: 4’ workbench with overhead task lighting, storage cabinets and drawers. The beamlines share countertop workspace around the perimeter, assembly table (60 in. X 32 in.), and beryllium storage cabinet.
Lab 3L-L05 is a dry lab that supports the beamline operation and user activities associated with the CHX, SMI, and CMS beamlines. The laboratory is mainly used for equipment setup & testing, sample preparation and characterization using complementary non-x-ray measurements prior to and during beam time, mechanical assembly of small-scale beamline components and sample chambers, and sample inspection. For motion control and inspection, the lab is equipped with a Delta Tau station and an optical micrometer* (Keyence). For electronic testing, the lab has a power supply (BK Precision), two function generators (BK Precision-4001 and Stanford), and an oscilloscope (Tektronix, TDS2014). For optical microscopy characterization of samples, the lab has a stereo microscope (Leica M125) and a polarizing microscope with epi- and transmission-illumination (Olympus BK51 Polarizing Microscopy, training required). The lab also has a water chiller/circulator (Neslab, Thermo Flex 900). The lab has shared workspace in the center and around the perimeter of the lab.
Lab 3L-L06 is a dry lab that is used for long term (months) requests for beamline and/or instrument assembly tasks. Requests to use this lab must be made well in advance of the time it will be needed.
The principle purpose of Lab 3L-L07 is wet chemistry. It is configured for environmental work with trace elements, toxic metals, and hazardous substances. As such, additional PPE (personal protection equipment) requirements may be required. Equipment available to users includes: 18.2 MegaOhm water source, glove box with dry nitrogen (N2) atmosphere*, orbital shaker, sample oven, analytical balance, high-capacity balance, digital pipettes, tabletop centrifuge, USDA standard sieves, pH electrodes and meters, sonicators, desiccators, freeze-drier*, vortexer, Leica stereomicroscope with digital camera, computer with internet access, fluorescent light bank for plant cultivation, cell culture hood, refrigerator*, circulator bath, hot/stir plates, lab glassware, lab jacks/stands and consumables (e.g., basic chemicals & reagents, LN2, gloves, goggles, Kimwipes, etc.). Reagent and LN2 use requires training.
The principle capabilities for Lab 3L-L08 include (1) sample preparation and (3) visible microscopy characterization for imaging and (3) a mammalian cell culture facility. For sample preparation, the lab is equipment with two Leica microtomes* (ambient and low temperature) and a Leica plunge freezer*. For visible microscopy characterization the lab has two upright stereo microscopes: a Nikon Eclipse microscope with bright-field transmission, epifluorescence, and dark field viewing modes, digital camera, and motorized stage; and an Olympus BX51 with bright-field & epifluorescence viewing modes and a digital camera. For mammalian cell culture*, the lab is equipped with a cell culture hood, incubator, autoclave, -80 freezer, LN2 storage dewar, and cytospin. br> *Requires training
|ROOM||LAB NAME||LAB PHONE||CONTACT (CSM)||CONTACT PHONE|
|199A||Laser Microspectroscopy Lab||631-344-3559||Babak Andi||631-344-3559|
The purpose of the microspectroscopy laboratory is to correlate electronic absorption and resonance Raman microspectroscopic measurements of single protein crystals with X-ray crystallography, scattering, and imaging. This lab is associated with NSLS-II crystallography and scattering beamlines (AMX, FMX, and LIX). Spectroscopic techniques can be used to elucidate the oxidation states of the metals in the proteins; to provide more insights into the orientations of the chromophores and/or ligands in a crystal; to detect reaction intermediates and in-crystallo enzyme assays and kinetics studies; to provide more insights into mysterious electron densities; and chemical bond vibrational studies. The lab instrumentations include: Four class-3b laser systems (473 nm, 532 nm, 633 nm, and 785 nm), iHR 550 Horiba Modular Raman Spectrometer with LabSpec 5.0 software, Ocean Optics USB4000 and QE65000 UV-Vis spectrometers (200-1000 nm), Crystal Logic diffractometer, Optique Peter Goniometer, Ocean Optics DH-2000 UV-VIS-NIR D2-hallogen light source and Hamamatsu L10290 High Power UV-Vis light source, Oxford cryosystems 700, Thermo Scientific NanoDrop 2000c UV-Vis spectrometer, Leica MZ8 microscope, and computer workstation.